GAP-JUNCTIONS IN THE RAT COCHLEA - IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL ANALYSIS
GAP-JUNCTIONS IN THE RAT COCHLEA - IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL ANALYSIS
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DOI：
10.1007/bf00186783
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发表时间：
1995-02-01
期刊：
影响因子：
--
通讯作者：
ADAMS, JC
中科院分区：
文献类型：
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作者：
KIKUCHI, T;KIMURA, RS;ADAMS, JC
用缝隙连接蛋白26免疫组织化学染色和透射电子显微镜观察大鼠耳蜗区缝隙连接。正常和预包埋免疫染色材料的电子显微镜显示，所有细胞之间存在缝隙连接，光镜显示这些细胞具有免疫染色的对位。光镜显示所有类型的细胞之间和之间的免疫染色，电子显微镜显示通过缝隙连接。因此，缝隙连接蛋白26的免疫染色被认为提供了整个耳蜗缝隙连接位置的合理近似值，并被用来提供这些位置的范围的概述。通过缝隙连接相互连接的细胞分为两组。第一组由无感觉上皮细胞组成，包括螺旋缘的牙间细胞、内沟细胞、Corti器支持细胞、外沟细胞和螺旋韧带根突内的细胞。第二组由结缔组织细胞组成，包括各种类型的螺旋缘和螺旋韧带的纤维细胞，血管纹的基细胞和中间细胞，以及排列在前庭阶的间充质细胞。本工作是对描述耳蜗细胞之间的连续缝隙连接如何反映组织的组织水平的第一次尝试。这里描述的组织，以及以前研究人员的大量信息表明，上皮细胞和结缔组织细胞的连续排列的缝隙连接是在转导过程中循环通过感觉细胞的内淋巴钾离子的结构基础。
Gap junctions in the rat cochlea were investigated using immunostaining for connexin26 and transmission electron microscopy. Electron microscopy of normal and pre-embedded immunostained material showed that there were gap junctions between and among all cells that light microscopy showed to have immunostained appositions. Light microscopy showed immunostaining between and among all cell types that electron microscopy showed to be joined by gap junctions. Immunostaining for connexin26 was therefore taken as providing a reasonable approximation of the locations of gap junctions throughout the cochlea and was used to provide an overview of the extent of those locations. Cells interconnected via gap junctions fell into one of two groups. The first group consists of nonsensory epithelial cells and includes interdental cells of the spiral limbus, inner sulcus cells, organ of Corti supporting cells, outer sulcus cells, and cells within the root processes of the spiral ligament. The second group consists of connective tissue cells and includes various fibrocyte types of the spiral limbus and spiral ligament, basal and intermediate cells of the stria vascularis, and mesenchymal cells which line the scala vestibuli. The present work represents a first attempt towards a description of how serial gap junctions among cochlear cells reflect a level of organization of the tissue. The organization described here, together with a great deal of information from previous investigators, suggest that serially arranged gap junctions of both epithelial and connective tissue cells serve as the strucural basis for recycling endolymphatic potassium ions that pass through the sensory cells during the transduction process.