Substitution of dietary ω-6 polyunsaturated fatty acids for saturated fatty acids decreases LDL apolipoprotein B-100 production rate in men with dyslipidemia associated with insulin resistance: a randomized controlled trial
Substitution of dietary ω-6 polyunsaturated fatty acids for saturated fatty acids decreases LDL apolipoprotein B-100 production rate in men with dyslipidemia associated with insulin resistance: a randomized controlled trial
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DOI：
10.1093/ajcn/nqx013
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发表时间：
2018-01-01
影响因子：
7.1
通讯作者：
Couture, Patrick
中科院分区：
文献类型：
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作者：
Drouin-Chartier, Jean-Philippe;Tremblay, Andre J.;Couture, Patrick
背景：在心血管疾病预防中提倡用omega (omega)-6 （n-6）多不饱和脂肪酸（PUFAs）代替饱和脂肪酸（sfa）。这种替代对与胰岛素抵抗（IR）相关的血脂异常受试者的脂蛋白代谢的影响尚不清楚。目的：在患有血脂异常和IR的男性中，我们评估了用omega-6 PUFAs代替SFAs对载脂蛋白（apo） b -脂蛋白体内动力学和参与脂蛋白代谢的关键基因肠道表达的影响。设计：招募血脂异常和IR男性（n = 36）参加这项双盲、随机、交叉、对照试验。受试者按随机顺序进食富含sfa的完全控制饮食（sfa: 13.4%的能量；omega-6 PUFAs: 4.0%）和富含omega-6 PUFAs的完全控制饮食（sfa: 6.0%; omega-6 PUFAs: 11.3%），为期4周，中间有4周的洗脱期。在每次饲粮结束时，测量含载脂蛋白的体内动力学，并在每个参与者的十二指肠活检中量化参与脂蛋白代谢的关键基因的肠道表达。结果：omega-6 PUFAs替代sfa对TRL apoB-48的分解代谢率（Delta = -3.8%， P= 0.7）和产率（Delta =+ 1.2%， P= 0.9）没有影响，尽管它下调了微粒体甘油三酯转移蛋白（Delta = -18.4%， P= 0.006）和载脂蛋白ob （Delta = -16.6%， P= 0.005）的肠道表达。用omega-6 PUFAs代替SFAs降低了LDL apoB-100池大小（δ =-7.8%； P= 0.005）。这一差异归因于用omega-6 PUFAs代替SFAs后LDL apoB-100生成速率的降低（δ = -10.0%; P = 0.003）。结论：本研究表明，在患有血脂异常和IR的男性中，用膳食中的omega-6 PUFAs代替sfa可以减少LDL颗粒的产生和数量。
Background: The substitution of omega (omega)-6 (n-6) polyunsaturated fatty acids (PUFAs) for saturated fatty acids (SFAs) is advocated in cardiovascular disease prevention. The impact of this substitution on lipoprotein metabolism in subjects with dyslipidemia associated with insulin resistance (IR) remains unknown.Objective: In men with dyslipidemia and IR, we evaluated the impact of substituting omega-6 PUFAs for SFAs on the in vivo kinetics of apolipoprotein (apo) B-containing lipoproteins and on the intestinal expression of key genes involved in lipoprotein metabolism.Design: Dyslipidemic and IR men (n = 36) were recruited for this double-blind, randomized, crossover, controlled trial. Subjects consumed, in a random order, a fully controlled diet rich in SFAs (SFAs: 13.4% of energy; omega-6 PUFAs: 4.0%) and a fully controlled diet rich in omega-6 PUFAs (SFAs: 6.0%; omega-6 PUFAs: 11.3%) for periods of 4 wk, separated by a 4-wk washout period. At the end of each diet, the in vivo kinetics of apoB-containing lipoproteins were measured and the intestinal expression of key genes involved in lipoproteinmetabolism was quantified in duodenal biopsies taken from each participant.Results: The substitution of omega-6 PUFAs for SFAs had no impact on TRL apoB-48 fractional catabolic rate (Delta = -3.8%, P = 0.7) and production rate (Delta =+ 1.2%, P= 0.9), although it downregulated the intestinal expression of the microsomal triglyceride transfer protein (Delta = -18.4%, P = 0.006) and apoB (Delta = -16.6%, P = 0.005). The substitution of omega-6 PUFAs for SFAs decreased the LDL apoB-100 pool size (Delta =-7.8%; P= 0.005). This difference was attributed to a reduction in the LDL apoB-100 production rate after the substitution of omega-6 PUFAs for SFAs (Delta = -10.0%; P = 0.003).Conclusions: This study demonstrates that the substitution of dietary omega-6 PUFAs for SFAs decreases the production and number of LDL particles in men with dyslipidemia and IR.