Structural analysis and DNA binding of the HMG domains of the human mitochondrial transcription factor A.
Structural analysis and DNA binding of the HMG domains of the human mitochondrial transcription factor A.
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DOI：
10.1093/nar/gkp157
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发表时间：
2009-06
影响因子：
14.9
通讯作者：
Churchill ME
中科院分区：
文献类型：
--
作者：
Gangelhoff TA;Mungalachetty PS;Nix JC;Churchill ME
线粒体转录因子A（mtTFA）是线粒体转录复合物组装和起始的核心。人mtTFA（h-mtTFA）是一种双重高迁移率族蛋白（HMGB），其与线粒体基因组位点特异性结合，并划分用于募集h-mtTFB 1、h-mtTFB 2和线粒体RNA聚合酶的启动子。的化学计量的h-mtTFA被发现是一个单体的情况下的DNA，而它形成的复合物中的轻链启动子（LSP）DNA的二聚体。评价每个HMG盒和C-末端尾与LSP DNA结合的能力。去除C-末端尾部仅略微降低非序列特异性DNA结合，并且盒A而不是盒B能够结合LSP DNA。在1.35 μ m分辨率下，h-mtTFA盒B的X射线晶体结构显示了非正则HMG盒的特征。观察到盒B与h-mtTFA的其他区域的相互作用。总之，这些结果提供了一个不寻常的盒B的DNA结合特性的解释，并建议在转录复合物组装这个域可能的作用。
The mitochondrial transcription factor A (mtTFA) is central to assembly and initiation of the mitochondrial transcription complex. Human mtTFA (h-mtTFA) is a dual high mobility group box (HMGB) protein that binds site-specifically to the mitochondrial genome and demarcates the promoters for recruitment of h-mtTFB1, h-mtTFB2 and the mitochondrial RNA polymerase. The stoichiometry of h-mtTFA was found to be a monomer in the absence of DNA, whereas it formed a dimer in the complex with the light strand promoter (LSP) DNA. Each of the HMG boxes and the C-terminal tail were evaluated for their ability to bind to the LSP DNA. Removal of the C-terminal tail only slightly decreased nonsequence specific DNA binding, and box A, but not box B, was capable of binding to the LSP DNA. The X-ray crystal structure of h-mtTFA box B, at 1.35 Å resolution, revealed the features of a noncanonical HMG box. Interactions of box B with other regions of h-mtTFA were observed. Together, these results provide an explanation for the unusual DNA-binding properties of box B and suggest possible roles for this domain in transcription complex assembly.
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DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
14.9
作者：
Klass, J;Murphy, FV;Churchill, MEA
通讯作者：
Churchill, MEA
影响因子：
7
作者：
Bogenhagen, DF;Wang, YS;Kobayashi, R
通讯作者：
Kobayashi, R
影响因子：
3.5
作者：
Ekstrand, MI;Falkenberg, M;Larsson, NG
通讯作者：
Larsson, NG
DOI：
10.1146/annurev.cb.07.110191.002321
发表时间：
1991-01-01
期刊：
ANNUAL REVIEW OF CELL BIOLOGY
影响因子：
--
作者：
CLAYTON, DA
通讯作者：
CLAYTON, DA