Constriction and Dnm1p recruitment are distinct processes in mitochondrial fission
Constriction and Dnm1p recruitment are distinct processes in mitochondrial fission
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DOI：
10.1091/mbc.e02-10-0657
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发表时间：
2003-05-01
影响因子：
3.3
通讯作者：
Kirchhausen, T
中科院分区：
文献类型：
--
作者：
Legesse-Miller, A;Massol, RH;Kirchhausen, T
线粒体经历了融合和分裂的循环，这对细胞器的动态平衡至关重要。分裂部分是通过将大的GTP酶Drun1p募集到线粒体膜外膜来调节的。利用酿酒酵母细胞的三维时间推移荧光成像，我们发现Dnm1p-EGFP出现和消失在沿线粒体管的热点。它形成的斑块可以迅速转换成不同的形状，无论随后是否会发生线粒体裂变。此外，线粒体基质的厚度呈现频繁的时间波动，显然与Dnm1p-EGFP的分裂或招募无关。这些结果表明，线粒体分裂需要至少两个不同的过程协调。
Mitochondria undergo cycles of fusion and fission crucial for organelle homeostasis. Fission is regulated partially by recruitment of the large GTPase Drun1p to the outer mitochondrial membrane. Using three-dimensional time-lapse fluorescence imaging of Saccharomyces cerevisiae cells, we found that Dnm1p-EGFP appears and disappears at "hot spots" along mitochondrial tubes. It forms patches that convert rapidly into different shapes regardless of whether mitochondrial fission ensues or not. Moreover, the thickness of the mitochondrial matrix displays frequent temporal fluctuations apparently unrelated to fission or to recruitment of Dnm1p-EGFP. These results suggest that mitochondrial fission requires coordination of at least two distinct processes.