Perylene-functionalized graphene sheets modified with chitosan for voltammetric discrimination of tryptophan enantiomers
Perylene-functionalized graphene sheets modified with chitosan for voltammetric discrimination of tryptophan enantiomers
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DOI：
10.1007/s00604-019-3442-5
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发表时间：
2019-05
影响因子：
5.7
通讯作者：
Xing Yang;Xiaohui Niu;Z. Mo;R. Guo;Nijuan Liu;P. Zhao;Zhenyu Liu
中科院分区：
文献类型：
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作者：
Xing Yang;Xiaohui Niu;Z. Mo;R. Guo;Nijuan Liu;P. Zhao;Zhenyu Liu
以3,4,9,10-苝四甲酸和壳聚糖功能化的石墨烯为原料，通过化学方法制备了复合材料（rGO-PTCA-壳聚糖）。它涉及用 PTCA 对 rGO 进行非共价官能化，然后与壳聚糖进行酰胺化反应。采用扫描电子显微镜、傅里叶变换红外光谱、X射线光电子能谱和电化学方法对复合材料进行了表征。通过结合壳聚糖的手性特征和rGO-PTCA优异的电化学行为，构建了一种具有对映选择性的石墨烯基材料，用于色氨酸（Trp）对映体的电化学手性识别。 rGO-PTCA-壳聚糖修饰的玻碳电极（GCE）对L-Trp的识别能力高于对D-Trp的识别能力。最佳工作电压接近 0.78 V（相对于 SCE），对映选择性系数为 3.0。该传感器在 1 mM 至 10 mM Trp 浓度范围内具有线性响应，L-Trp 的检测限为 1.2 μM（S/N= 3），D-Trp 的检测限为 3.0 μM。该传感器成功用于检测实际样品中的Trp对映体，并提出了识别机制。图文摘要通过化学方法制备了3,4,9,10-苝四甲酸和壳聚糖功能化石墨烯复合物（rGO-PTCA-壳聚糖）的示意图。它涉及用 PTCA 对 rGO 进行非共价官能化，然后与壳聚糖进行酰胺化反应，并伏安法测定色氨酸对映体。
A composite was prepared from graphene functionalized with 3,4,9,10-perylene tetracarboxylic acid and chitosan (rGO-PTCA-chitosan) by a chemical method. It involves non-covalent functionalization of rGO with PTCA followed by amidation reaction with chitosan. Scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and electrochemical methods were used to characterize the composites. By combining the chiral features of chitosan and the excellent electrochemical behaviors of rGO-PTCA, a graphene-based material with enantioselectivity was constructed for electrochemical chiral recognition of tryptophan (Trp) enantiomers. A glassy carbon electrode (GCE) modified with rGO-PTCA-chitosan had a higher recognition capability for L-Trp than for D-Trp. Best operated at a working voltage near 0.78 V (vs. SCE), the enantioselectivity coefficient is 3.0. The sensor has a linear response in the 1 mM to 10 mM Trp concentration range and a 1.2 μM detection limit (at S/N= 3) for L-Trp, and of 3.0 μM to D-Trp. The sensor was successfully used to detect Trp enantiomers in real samples, and a recognition mechanism is presented.Graphical abstractSchematic presentation of a composoie prepared by graphene functionalized with 3,4,9,10-perylene tetracarboxylic acid and chitosan (rGO-PTCA-chitosan) via a chemical method. It involves non-covalent functionalization of rGO with PTCA followed by amidation reaction with chitosan and voltammetric determination of tryptophan enantiomers.