Two-dimensional tracking of a motile micro-organism allowing high-resolution observation with various imaging techniques
Two-dimensional tracking of a motile micro-organism allowing high-resolution observation with various imaging techniques
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DOI：
10.1063/1.1857632
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发表时间：
2005-03-01
影响因子：
1.6
通讯作者：
Hashimoto, K
中科院分区：
文献类型：
--
作者：
Oku, H;Ogawa, N;Hashimoto, K
In this article, a micro-organism tracking system using a high-speed vision system is reported. This system two dimensionally tracks a freely swimming micro-organism within the field of an optical microscope by moving a chamber of target micro-organisms based on high-speed visual feedback. The system we developed could track a paramecium using various imaging techniques, including bright-field illumination, dark-field illumination, and differential interference contrast, at magnifications of 5 times and 20 times. A maximum tracking duration of 300 s was demonstrated. Also, the system could track an object with a velocity of up to 35 000 mu m/s (175 diameters/s), which is significantly faster than swimming micro-organisms. (C) 2005 American Institute of Physics.
