Induction of humoral and cellular immune responses against the nucleocapsid of bovine viral diarrhea virus by an adenovirus vector with an inducible promoter
Induction of humoral and cellular immune responses against the nucleocapsid of bovine viral diarrhea virus by an adenovirus vector with an inducible promoter
复制标题
DOI：
10.1006/viro.1999.9828
复制
发表时间：
1999-08-15
期刊：
影响因子：
3.7
通讯作者：
Elazhary, Y
中科院分区：
文献类型：
--
作者：
Elahi, SM;Shen, SH;Elazhary, Y
构建了一种新的重组腺病毒，其在四环素可调控启动子的控制下表达牛病毒性腹泻病毒（BVDV）的核衣壳（C蛋白或p14）。用该重组腺病毒与另一种表达反式激活蛋白的重组腺病毒共接种小鼠，诱导了对BVDV/C蛋白的强烈体液免疫应答，如ELISA所检测。用重组腺病毒免疫的小鼠的脾细胞显示对BVDV的两种基因型（1型和2型）的特异性增殖反应。当在体外用两种基因型BVDV刺激时，在重组腺病毒免疫的小鼠的鼠单核细胞上清液中检测到高水平的IFN-γ。这些结果表明，该重组腺病毒具有高度免疫原性，并刺激针对BVDV核衣壳的细胞和体液免疫应答。(C)北京：科学出版社.
A new recombinant adenovirus was constructed that expressed the nucleocapsid (C protein or p14) of the bovine viral diarrhea virus (BVDV) under the control of a tetracycline-regulatable promoter. Mice covaccinated with this recombinant adenovirus, accompanied by another recombinant adenovirus expressing the trans-activator protein, induced a strong humoral immune response to the BVDV/C protein as detected by ELISA. Splenocytes from mice immunized with the recombinant adenovirus showed a specific proliferation response to both genotypes (type 1 and 2) of BVDV. High levels of IFN-gamma were detected in the supernatant of murine mononuclear cells of mice immunized by the recombinant adenovirus when stimulated in vitro by both genotypes of BVDV. These results indicate that this recombinant adenovirus is highly immunogenic and stimulates both cellular and humoral immune responses against the nucleocapsid of BVDV. (C) 1999 Academic Press.