Engineering artificial cells by combining HeLa-based cell-free expression and ultrathin double emulsion template.
Engineering artificial cells by combining HeLa-based cell-free expression and ultrathin double emulsion template.
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DOI：
10.1016/bs.mcb.2015.01.014
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发表时间：
2015
影响因子：
--
通讯作者：
Liu AP
中科院分区：
文献类型：
--
作者：
Ho KK;Murray VL;Liu AP
人工细胞的产生为研究整个细胞中生物过程的复杂性和在体外重建系统中研究这些过程之间的差距提供了桥梁。人工细胞被定义为将生物活性物质封装在生物或合成膜中。在这里，我们描述了一种鲁棒和通用的方法来产生人造细胞，以模仿细胞的一种或多种行为。采用微流控双乳系统封装了一种能够将膜蛋白表达到双分子层或囊泡内可溶性蛋白的哺乳动物细胞自由表达系统。开发一个强大的平台，允许人工细胞的组装，对于理解亚细胞功能和在更类似细胞的环境中的紧急行为，以及创建新的信号通路来实现特定的细胞行为是有价值的。
Generation of artificial cells provides the bridge needed to cover the gap between studying the complexity of biological processes in whole cells and studying these same processes in an in vitro reconstituted system. Artificial cells are defined as the encapsulation of biologically active material in a biological or synthetic membrane. Here, we describe a robust and general method to produce artificial cells for the purpose of mimicking one or more behaviors of a cell. A microfluidic double emulsion system is used to encapsulate a mammalian cell free expression system that is able to express membrane proteins into the bilayer or soluble proteins inside the vesicles. The development of a robust platform that allows the assembly of artificial cells is valuable in understanding subcellular functions and emergent behaviors in a more cell-like environment as well as for creating novel signaling pathways to achieve specific cellular behaviors.