Overexpression of annexin V in cystic fibrosis epithelial cells from fetal trachea.
Overexpression of annexin V in cystic fibrosis epithelial cells from fetal trachea.
复制标题
胎儿气管囊性纤维化上皮细胞中膜联蛋白 V 的过度表达。
DOI：
10.1006/excr.1995.1242
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发表时间：
1995
影响因子：
3.7
通讯作者：
Gruenert,DC
中科院分区：
文献类型：
--
作者：
DellaGaspera,B;Weinman,S;Huber,C;Lemnaouar,M;Paul,A;Picard,J;Gruenert,DC
在这份报告中，我们用Northern和Western印迹分析了从人胎儿气管分离的四个细胞系中膜联蛋白I、II、V和VI的表达。其中两个细胞系取自正常胎儿，另两个细胞系取自囊性纤维化胎儿。1例CF型胎儿为S549N和N1303K杂合子，另1例为ΔF508缺失纯合子。我们发现这四种膜联蛋白总是共表达的。在四种细胞系中，膜联蛋白I、II和VI的表达相同。而在正常细胞中，膜联蛋白V的表达明显高于正常细胞。这些观察表明，在气管上皮细胞系中，膜联蛋白I、II、V和VI是独立调节的。此外，他们认为，过表达的膜联蛋白V是一种钙离子通道，可能会深刻地改变CF细胞膜上的钙离子转运。
In this report, we investigated the expression of annexins I, II, V, and VI by Northern and Western blot analysis in four cell lines isolated from human fetal tracheae. Two cell lines were obtained from normal fetuses and the two others from fetuses with cystic fibrosis (CF). One CF fetus was heterozygous for the S549N and N1303K substitutions, whereas the other was homozygous for the ΔF508 deletion. We found that the four annexins are always coexpressed. The expression of annexins I, II, and VI was the same in the four cell lines. In contrast, that of annexin V was significantly higher in CF than in normal cells. These observations demonstrate that annexins I, II, V, and VI are independently regulated in tracheal epithelial cell lines. Moreover, they suggest that the overexpressed annexin V, a Ca2+channel, might profoundly modify Ca2+transport across the membranes of CF cells.