An insect virus-encoded microRNA regulates viral replication
An insect virus-encoded microRNA regulates viral replication
复制标题
DOI：
10.1128/jvi.01109-08
复制
发表时间：
2008-09-01
影响因子：
5.4
通讯作者：
Asgari, Sassan
中科院分区：
文献类型：
--
作者：
Hussain, Mazhar;Taft, Ryan J.;Asgari, Sassan
MicroRNAs(MiRNAs)是一种小的(类似于22个核苷酸的)非编码RNA，在基因调控中发挥重要作用，并影响包括发育、分化和肿瘤发生在内的广泛过程。在这里，我们报告了第一个从昆虫病毒中分离到的miRNA，它来自于绿色日光夜蛾病毒(HvAV)的主要衣壳蛋白(MCP)基因(HvAV-miR-1)。虽然MCP在感染后24 h的各个时间点都有大量表达，但HvAV-miR-1的表达从感染后96 h开始受到严格调控并特异性检测到。HvAV-miR-1的表达与HvAV DNA聚合酶I的表达显著降低相一致，HvAV DNA聚合酶I是一个预测的目标。保留miRNA序列的MCP全长和截短版本的异位表达显著降低了DNA聚合酶I的转录水平，并抑制了病毒的复制。我们的结果表明，HvAV-miR-1指导DNA聚合酶I的转录降解，并调节HvAV的复制。这些发现与最近关于miR-BART-2调节Epstein-Barr病毒DNA聚合酶表达的报道是一致的，并提示病毒编码的miRNA对病毒复制的调节可能是一个普遍现象。
MicroRNAs (miRNAs) are small (similar to 22 nucleotides) noncoding RNAs which play an essential role in gene regulation and affect a wide range of processes, including development, differentiation, and oncogenesis. Here we report the identification of the first miRNA from an insect virus, derived from the major capsid protein (MCP) gene in Heliothis virescens ascovirus (HvAV) (HvAV-miR-1). Although MCP was abundantly expressed at all time points 24 h after infection, HvAV-miR-1 expression was strictly regulated and specifically detected from 96 h postinfection. HvAV-miR-1 expression coincided with a marked reduction of the expression of HvAV DNA polymerase I, which is a predicted target. Ectopic expression of full- length and truncated versions of MCP retaining the miRNA sequence significantly reduced DNA polymerase I transcript levels and inhibited viral replication. Our results indicate that HvAV-miR-1 directs transcriptional degradation of DNA polymerase I and regulates HvAV replication. These findings are congruent with recent reports that miR-BART-2 regulates Epstein-Barr virus DNA polymerase expression and suggest that virus-encoded miRNA regulation of virus replication may be a general phenomenon.