Functional specialization of duplicated AP3-like genes in Medicago truncatula
Functional specialization of duplicated AP3-like genes in Medicago truncatula
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DOI：
10.1111/tpj.12068
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发表时间：
2013-02-01
期刊：
影响因子：
7.2
通讯作者：
Canas, Luis A.
中科院分区：
文献类型：
--
作者：
Roque, Edelin;Serwatowska, Joanna;Canas, Luis A.
MADS盒基因的B类已经在广泛的植物物种中进行了研究，但在豆类中仍然很大程度上未被表征。在这里，我们调查的重复AP3样基因的豆科植物物种的进化命运。为了深入了解B类MADS盒基因功能在豆科植物中保守或多样化的程度，我们分离并表征了蒺藜苜蓿中AP3谱系的两个成员：MtNMH7和MtTM6（分别为euAP3和paleoAP3基因）。两个基因在花瓣和雄蕊中的表达模式不重叠且互补。MtTM 6主要表达于两种花器官的外细胞层，MtNMH 7主要表达于花瓣和雄蕊的内细胞层。通过反向遗传学方法（RNAi和Tnt 1诱变）进行的功能分析表明，MtNMH 7对花瓣身份的贡献比MtTM 6更重要，而MtTM 6在雄蕊身份中起着比其旁系MtNMH 7更重要的作用。我们的研究结果表明，蒺藜苜蓿AP3样基因经历了一个功能特化过程与祖先基因谱系的基因表达模式的完全分区。我们提供了关于核心真双子叶植物之间花瓣和雄蕊发育的相似性和差异性的信息。
The Bclass of MADS box genes has been studied in a wide range of plant species, but has remained largely uncharacterized in legumes. Here we investigate the evolutionary fate of the duplicated AP3-like genes of a legume species. To obtain insight into the extent to which B-class MADS box gene functions are conserved or have diversified in legumes, we isolated and characterized the two members of the AP3 lineage in Medicago truncatula: MtNMH7 and MtTM6 (euAP3 and paleoAP3 genes, respectively). A non-overlapping and complementary expression pattern of both genes was observed in petals and stamens. MtTM6 was expressed predominantly in the outer cell layers of both floral organs, and MtNMH7 in the inner cell layers of petals and stamens. Functional analyses by reverse genetics approaches (RNAi and Tnt1 mutagenesis) showed that the contribution of MtNMH7 to petal identity is more important than that of MtTM6, whereas MtTM6 plays a more important role in stamen identity than its paralog MtNMH7. Our results suggest that the M.truncatula AP3-like genes have undergone a functional specialization process associated with complete partitioning of gene expression patterns of the ancestral gene lineage. We provide information regarding the similarities and differences in petal and stamen development among core eudicots.