Functional Analysis of Brain-Engrafted Monocytes After Microglia Ablation in Mouse Models.
Functional Analysis of Brain-Engrafted Monocytes After Microglia Ablation in Mouse Models.
复制标题
小鼠模型中小胶质细胞消融后脑移植单核细胞的功能分析。
DOI：
10.1007/978-1-4939-9658-2_22
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Neher,JonasJ
中科院分区：
文献类型：
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作者：
Varvel,NicholasH;Ransohoff,RichardM;Neher,JonasJ
在侮辱和疾病期间，血液传播的单核细胞可以侵入大脑和脊髓，与驻留在大脑中的小胶质细胞一起促进神经免疫反应。由于这两种免疫细胞的共同标志物表达和形态，脑浸润性单核细胞的特定功能一直难以确定。在这里，我们描述了在小胶质细胞消融后用循环单核细胞重新填充大脑的方法，以研究在这些条件下移植的脑侵袭单核细胞的生理学。
During insults and disease blood-borne monocytes can invade brain and spinal cord, contributing to the neuroimmune response together with brain-resident microglia. The specific function of brain-infiltrating monocytes has been difficult to ascertain because of shared marker expression and morphology of these two immune cell types. Here we describe our method of repopulating the brain with circulating monocytes after microglia ablation to investigate the physiology of brain-invading monocytes, which engraft under these conditions.