IMPROVED METHODOLOGY FOR ANALYSIS AND QUANTITATION OF PROTEINS ON ONE-DIMENSIONAL SILVER-STAINED SLAB GELS
IMPROVED METHODOLOGY FOR ANALYSIS AND QUANTITATION OF PROTEINS ON ONE-DIMENSIONAL SILVER-STAINED SLAB GELS
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DOI：
10.1016/0003-2697(83)90551-1
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
GREASER, M
中科院分区：
文献类型：
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作者：
GIULIAN, GG;MOSS, RL;GREASER, M
描述了一种用于分离和定量来自单个[兔]肌纤维的低分子量（75-10 kD [千道尔顿]）蛋白质的十二烷基硫酸钠-不连续聚丙烯酰胺凝胶电泳系统。平板凝胶（0.75 mm厚）使用改进的Ag染色技术进行染色，该技术不需要照相定影剂来实现低水平背景染色。改良的染色程序使用连续流动洗涤，以最大限度地减少凝胶处理。该方法具有高灵敏度，并给出了在λ之间的线性响应。2和70 ng蛋白质/条带。还开发了一种方便的方法来安装平板凝胶，用于摄影、扫描和长期储存。
A sodium dodecyl sulfate-discontinuous polyacrylamide gel electrophoresis system for separation and quantitation of low MW (75-10 kD [kilodalton]) proteins from single [rabbit] muscle fibers is described. Slab gels (0.75 mm thick) were stained using an improved Ag-stain technique which does not require photographic fixers to achieve low-level background staining. The modified staining procedure uses continuous flow washing to minimize the handling of gels. The procedure has high sensitivity and gave a linear response between .apprx. 2 and 70 ng of protein per band. A convenient method for mounting slab gels for photography, scanning and long-term storage was also developed.