Gene expression profiling of pre-eclamptic placentae by RNA sequencing.
Gene expression profiling of pre-eclamptic placentae by RNA sequencing.
复制标题
DOI：
10.1038/srep14107
复制
发表时间：
2015-09-21
影响因子：
4.6
通讯作者：
FINNPEC Core Investigator Group
中科院分区：
文献类型：
--
作者：
Kaartokallio T;Cervera A;Kyllönen A;Laivuori K;Kere J;Laivuori H;FINNPEC Core Investigator Group
先兆子痫是一种常见且复杂的妊娠疾病，通常涉及胎盘发育受损。为了确定先兆子痫胎盘中改变的基因表达，我们对9名先兆子痫和9名健康孕妇的胎盘转录组进行了测序。通过在分析中包括所有池和基于表型特征排除一些池来测试差异基因表达。从这些分析中，我们共鉴定了53个不同表达的基因，其中一个子集在20例病例和19例对照中通过qPCR进行了验证。此外，我们进行了途径和功能分析，揭示了先兆子痫胎盘血管功能和免疫平衡紊乱。在我们的研究中发现的一些基因已经被许多微阵列研究报道（BHLHE 40、FSTL 3、HK 2、HTRA 4、LEP、PVRL 4、SASH 1、SIGLEC 6），但许多基因仅在少数研究中涉及，或以前未与先兆子痫相关（ARMS2、BTNL 9、CCSAP、DIO2、FER1L4、HPSE、LOC 100129345、林恩、MYO 7 B、NCMAP、NDRG 1、NRIP 1、PLIN 2、SBSPON、SERPINB 9、SH 3BP 5、TET 3、TPBG、ZNF 175）。由这些基因产生的几种分子可能在先兆子痫的发病机制中起作用，其中一些可以作为预测或检测这种妊娠并发症的生物标志物。
Pre-eclampsia is a common and complex pregnancy disorder that often involves impaired placental development. In order to identify altered gene expression in pre-eclamptic placenta, we sequenced placental transcriptomes of nine pre-eclamptic and nine healthy pregnant women in pools of three. The differential gene expression was tested both by including all the pools in the analysis and by excluding some of the pools based on phenotypic characteristics. From these analyses, we identified altogether 53 differently expressed genes, a subset of which was validated by qPCR in 20 cases and 19 controls. Furthermore, we conducted pathway and functional analyses which revealed disturbed vascular function and immunological balance in pre-eclamptic placenta. Some of the genes identified in our study have been reported by numerous microarray studies (BHLHE40, FSTL3, HK2, HTRA4, LEP, PVRL4, SASH1, SIGLEC6), but many have been implicated in only few studies or have not previously been linked to pre-eclampsia (ARMS2, BTNL9, CCSAP, DIO2, FER1L4, HPSE, LOC100129345, LYN, MYO7B, NCMAP, NDRG1, NRIP1, PLIN2, SBSPON, SERPINB9, SH3BP5, TET3, TPBG, ZNF175). Several of the molecules produced by these genes may have a role in the pathogenesis of pre-eclampsia, and some could qualify as biomarkers for prediction or detection of this pregnancy complication.