Temporally coordinated assembly and disassembly of replication factories in the absence of DNA synthesis
Temporally coordinated assembly and disassembly of replication factories in the absence of DNA synthesis
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DOI：
10.1038/35036309
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发表时间：
2000-10-01
影响因子：
21.3
通讯作者：
Gilbert, DM
中科院分区：
文献类型：
--
作者：
Dimitrova, DS;Gilbert, DM
在这里，我们表明，暴露于蛋白激酶抑制剂5-氨基嘌呤或咖啡因的aphidicolin-逮捕中国仓鼠卵巢（CHO）细胞的结果开始复制在连续后期复制染色体结构域，丧失能力，合成DNA在早期复制网站，释放Mcm 2蛋白质从染色质，以及在没有可检测到的复制叉延长的情况下，PCNA和RPA从早期复制域到晚期复制域的重新分布。这些结果提供的证据表明，在复制压力的条件下，检查点控制不仅可以防止进一步启动，但也可能需要积极保持停滞的复制复合物的完整性。
Here we show that exposure of aphidicolin-arrested Chinese hamster ovary (CHO) cells to the protein-kinase inhibitors 5-aminopurine or caffeine results in initiation of replication at successively later-replicating chromosomal domains, loss of the capacity to synthesize DNA at earlier-replicating sites, release of Mcm2 proteins from chromatin, and redistribution of PCNA and RPA from early- to late-replicating domains in the absence of detectable elongation of replication forks. These results provide evidence that, under conditions of replicational stress, checkpoint controls not only prevent further initiation but may also be required to actively maintain the integrity of stalled replication complexes.