Discrimination of Major Capsular Types of Campylobacter jejuni by Multiplex PCR
Discrimination of Major Capsular Types of Campylobacter jejuni by Multiplex PCR
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DOI：
10.1128/jcm.02348-10
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发表时间：
2011-05-01
影响因子：
9.4
通讯作者：
Guerry, Patricia
中科院分区：
文献类型：
--
作者：
Poly, Frederic;Serichatalergs, Oralak;Guerry, Patricia
空肠弯曲杆菌的多糖胶囊（CPS）是Penner血清分型方案的主要血清决定因素。空肠梭菌共有47种Penner血清型，其中22种为相关血清型的复合体。建立了一种比传统的Penner分型更简单、更经济的空肠弯曲杆菌荚膜类型多重PCR检测方法。根据本研究测序的8个主要血清型菌株的可变胶囊位点基因序列数据库和10个已发表的其他血清型菌株的基因序列，设计各胶囊型特异性引物。DNA序列分析显示，荚膜基因座具有镶嵌性质，表明基因通过水平转移进行了重组，并证明了Penner复合物内基因的高度保守性。使用244株已知的Penner型菌株，多重PCR可在两种PCR中区分17种单独的血清型，敏感性和特异性从90%到100%不等。
The polysaccharide capsule (CPS) of Campylobacter jejuni is the major serodeterminant of the Penner serotyping scheme. There are 47 Penner serotypes of C. jejuni, 22 of which fall into complexes of related serotypes. A multiplex PCR method for determination of capsule types of Campylobacter jejuni which is simpler and more affordable than classical Penner typing was developed. Primers specific for each capsule type were designed on the basis of a database of gene sequences from the variable capsule loci of 8 strains of major serotypes sequenced in this study and 10 published sequences of other serotypes. DNA sequence analysis revealed a mosaic nature of the capsule loci, suggesting reassortment of genes by horizontal transfer, and demonstrated a high degree of conservation of genes within Penner complexes. The multiplex PCR can distinguish 17 individual serotypes in two PCRs with sensitivities and specificities ranging from 90 to 100% using 244 strains of known Penner type.