Toll-like receptors, NOD1, and NOD2 in oral epithelial cells
Toll-like receptors, NOD1, and NOD2 in oral epithelial cells
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DOI：
10.1177/154405910608500609
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发表时间：
2006-06-01
影响因子：
7.6
通讯作者：
Takada, H.
中科院分区：
文献类型：
--
作者：
Sugawara, Y.;Uehara, A.;Takada, H.
口腔上皮可能是牙周组织抵抗口腔细菌的第一道屏障。我们假设，口腔上皮细胞被赋予先天性免疫受体的细菌成分，发挥作用，在宿主防御细菌感染，而不伴随过度的炎症反应。免疫组化结果显示，Toll样受体（TLR）4和TLR 2在正常口腔黏膜上皮组织中表达明显，NOD 1和NOD 2在正常口腔黏膜上皮组织中强表达。我们还发现，原代口腔上皮细胞在文化中表达这些分子，用PCR，流式细胞仪，免疫染色。在发炎的口腔上皮中，TLR 2和TLR 4的细胞表面定位比在健康组织中更清楚地观察到。在用这些受体的合成配体刺激后，β-防御素2的表达显著上调。这些发现表明，口腔上皮细胞中的这些分子是诱导抗菌反应的功能性受体。
Oral epithelium might be the first barrier against oral bacteria in periodontal tissue. We hypothesized that oral epithelium is endowed with innate immune receptors for bacterial components, which play roles in host defense against bacterial infection without being accompanied by excessive inflammatory responses. We found clear expression of Toll-like receptor (TLR) 4 as well as TLR2, and strong expression of NOD1 and NOD2 in normal oral epithelial tissues by immunohistochemical analysis. We also showed that primary oral epithelial cells in culture expressed these molecules using PCR, flow cytometry, and immunostaining. In inflamed oral epithelium, cell-surface localizations of TLR2 and TLR4 were more clearly observed than in healthy tissue. Upon stimulation with synthetic ligands for these receptors, the expression of beta-defensin 2 was markedly up-regulated. These findings indicate that these molecules in oral epithelial cells are functional receptors that induce antibacterial responses.