Salsolinol is a Putative Endogenous Neuro‐intermediate Lobe Prolactin‐Releasing Factor
Salsolinol is a Putative Endogenous Neuro‐intermediate Lobe Prolactin‐Releasing Factor
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Salsolinol 是一种假定的内源性神经中叶催乳素释放因子
DOI：
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发表时间：
2001
影响因子：
3.2
通讯作者：
G. Nagy
中科院分区：
文献类型：
--
作者：
B. Tóth;K. Homicsko;B. Radnai;W. Maruyama;J. E. Demaria;M. Vecsernyés;M. Fekete;F. Fülöp;M. Naoi;M. Freeman;G. Nagy
从脑垂体神经中间叶分离和鉴定催乳素释放因子（PRF）已经进行了十多年的研究。使用高压液相色谱-电化学检测（HPLC - ECD）和气相色谱/质谱（GC/MS） (R) - salsolinol (SAL)（一种多巴胺相关的立体特异性四氢异喹啉）被发现存在于雄性、完整和去卵巢的雌性大鼠的神经中间叶和正中突起提取物中。此外，对神经中间叶SAL浓度的分析显示，在分离4小时后短暂（10分钟）的哺乳刺激下，泌乳大鼠血浆泌乳素水平平行增加。SAL在体内似乎是一种选择性和有效的催乳素刺激剂，而对其他垂体激素的分泌没有影响。我们还发现，SAL可以提高泌乳素的释放，尽管在垂体细胞培养和肾包膜下前叶移植的垂体切除大鼠中，其作用程度较低。SAL对[3H] - spiperone与AP匀浆结合缺乏干扰，表明SAL不作用于多巴胺D2受体。此外，[3H]‐SAL特异性结合从哺乳期大鼠获得的AL匀浆和神经中间体叶。综上所述，我们的数据清楚地表明，SAL是原位合成的，这种化合物可以在调节垂体催乳素分泌中发挥作用。
The isolation and identification of a prolactin‐releasing factor (PRF) from the neuro‐intermediate lobe of the pituitary gland has been pursued for over a decade. Using high‐pressure liquid chromatography with electrochemical detection (HPLC‐ECD) and gas chromatography/mass spectrometry (GC/MS) (R)‐salsolinol (SAL) (a dopamine‐related stereo‐specific tetrahydroisoquinoline) was found to be present in neuro‐intermediate lobe as well as median eminence extracts of male, intact‐, and ovariectomized female rats. Moreover, analysis of SAL concentrations in neuro‐intermediate lobe revealed parallel increases with plasma prolactin in lactating rats exposed to a brief (10 min) suckling stimulus following 4‐h separation. SAL appears to be a selective and potent stimulator of prolactin secretion in vivo and it was without effect on the secretion of other pituitary hormones. We have also found that SAL can elevate prolactin release, although to a lesser extent, in pituitary cell cultures as well as in hypophysectomized rats bearing anterior lobe transplants under the kidney capsule. Lack of interference of SAL with [3H]‐spiperone binding to AP homogenates indicates that SAL does not act at the dopamine D2 receptor. Moreover, [3H]‐SAL binds specifically to homogenate of AL as well as neuro‐intermediate lobe obtained from lactating rats. Taken together, our data clearly suggest that SAL is synthesized in situ and this compound can play a role in the regulation of pituitary prolactin secretion.
垂体后叶含有有效的催乳素释放因子：体内研究。
影响因子：
4.8
作者：
Hyde,JF;Ben-Jonathan,N
通讯作者：
Ben-Jonathan,N
影响因子：
4.8
作者：
Hyde,JF;Ben-Jonathan,N
通讯作者：
Ben-Jonathan,N