The transition of early translocation intermediates in chloroplasts is accompanied by the movement of the targeting signal on the precursor protein.
The transition of early translocation intermediates in chloroplasts is accompanied by the movement of the targeting signal on the precursor protein.
复制标题
叶绿体中早期易位中间体的转变伴随着前体蛋白上靶向信号的移动。
DOI：
10.1016/j.abb.2008.06.007
复制
发表时间：
2008
影响因子：
3.9
通讯作者：
M. Akita
中科院分区：
文献类型：
--
作者：
H. Inoue;M. Akita
在蛋白质进入叶绿体的过程中，前体蛋白在严格的能量条件下与这些细胞器对接，形成早期的易位中间体。根据温度和对ATP的需求，存在不同类型的早期中间体，其前体蛋白转运的程度不同[H. Inoue，M.秋田，生物化学杂志283（2008）7491-7502]。然而，尚未确定前体周围的环境是否因每种中间体而不同。因此，我们在当前的研究中采用了位点特异性光交联策略，以捕获早期中间体中紧邻前体靶向信号的任何组分。鉴定了各种交联产物，其中一种含有Toc 75。发现这些产物的外观取决于交联剂改性后前体的位置以及中间状态。这表明早期易位中间体的转变伴随着早期中间体中靶向信号的移动。
During protein import into chloroplasts, precursor proteins are docked to these organelles under stringent energy conditions to form early translocation intermediates. Depending on the temperature and the requirement for ATP, different types of early-intermediates are present, for which the extent of precursor protein translocation differs [H. Inoue, M. Akita, J. Biol. Chem. 283 (2008) 7491–7502]. However, it has not been determined whether the environment surrounding the precursor differs for each intermediate. We therefore employed a site-specific photo-crosslinking strategy in our current study to capture any components in close proximity to the targeting signal of the precursors within the early-intermediates. Various crosslinked products, one of which contains Toc75, were identified. The appearance of these products was found to be dependent on the position of the precursor upon modification by the crosslinker and also the intermediate state. This indicated that the transition of early translocation intermediates is accompanied with the movement of the targeting signal within the early-intermediates.
影响因子：
2.9
作者：
DORMAN, G;PRESTWICH, GD
通讯作者：
PRESTWICH, GD
影响因子：
3.3
作者：
Becker, T;Hritz, J;Schleiff, E
通讯作者：
Schleiff, E