Enhanced MTT-reducing activity under growth inhibition by resveratrol in CEM-C7H2 lymphocytic leukemia cells
Enhanced MTT-reducing activity under growth inhibition by resveratrol in CEM-C7H2 lymphocytic leukemia cells
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DOI：
10.1016/s0304-3835(03)00157-5
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发表时间：
2003-06-10
期刊：
影响因子：
9.7
通讯作者：
Csordas, A
中科院分区：
文献类型：
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作者：
Bernhard, D;Schwaiger, W;Csordas, A
白藜芦醇对CEM-C7H2淋巴细胞白血病细胞增殖的抑制作用与增强细胞3-[4,5-二甲基二唑-2-基]-2,5-二苯基溴化四唑（MTT）还原活性相关联。这种现象在5 ~ 20 μ m白藜芦醇亚凋亡浓度范围内最为明显。我们的研究结果表明，多酚类抗氧化剂白藜芦醇可以增加mtt -还原活性，而不增加活细胞的数量，并且这种活性发生在抗氧化剂的浓度范围内，该浓度范围不足以诱导细胞凋亡，但足以减缓细胞生长。这种现象似乎仅限于具有抗氧化特性的增殖抑制剂，并且是细胞类型特异性的。因此，在确定类黄酮和多酚对增殖的影响时，在某些细胞类型中，这可能代表了MTT增殖试验中的一个缺陷。(C) 2003爱思唯尔科学爱尔兰有限公司版权所有。
Inhibition of proliferation by resveratrol of CEM-C7H2 lymphocytic leukemia cells was paradoxically associated with an enhanced cellular 3-[4,5-dimethyltbiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)-reducing activity. This phenomenon was most pronounced at the sub-apoptotic concentration range of 5-20 muM resveratrol. The results of our study show that the MTT-reducing activity can be increased by the polyphenolic antioxidant resveratrol without a corresponding increase in the number of living cells and that this occurs at a concentration range of the antioxidant which is not sufficient to induce apoptosis but suffices to slow down cell growth. This phenomenon appears to be restricted to proliferation inhibitors with antioxidant properties and is cell type-specific. Thus, in determining the effects of flavonoids and polyphenols on proliferation, in certain cell types this might represent a pitfall in the MTT proliferation assay. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.