Two-photon graphene oxide/aptamer nanosensing conjugate for in vitro or in vivo molecular probing.
Two-photon graphene oxide/aptamer nanosensing conjugate for in vitro or in vivo molecular probing.
复制标题
DOI：
10.1021/ac5000015
复制
发表时间：
2014-03
影响因子：
7.4
通讯作者：
Mei Yi;Sheng Yang;Zanying Peng;Changhui Liu;Jishan Li;Wenwan Zhong;Ronghua Yang;W. Tan
中科院分区：
文献类型：
--
作者：
Mei Yi;Sheng Yang;Zanying Peng;Changhui Liu;Jishan Li;Wenwan Zhong;Ronghua Yang;W. Tan
以近红外光子为激发源的双光子激发具有组织自荧光和自吸收低、光损伤和光漂白减少、空间分辨率高、穿透深度大(>；500μm)等独特性能。以氧化石墨烯(GO)为代表的碳纳米材料具有良好的生物相容性、高效的细胞内转运蛋白、保护携带的DNA或多肽不被酶切割、以及超荧光猝灭效率等优点。将纳米碳材料与热塑性弹性体技术相结合，设计了一种适体-双光子染料/GO热塑性弹性体荧光纳米传感偶联物，用于生物体液、活细胞和斑马鱼的分子探测。这种方法利用了GO对接近的TP染料具有特殊的猝灭能力，以及单链DNA对GO的亲和力高于适体-靶复合体。该传感策略成功地在体外和体内检测到了三磷酸腺苷。我们的结果表明，GO/适体-TP染料体系不仅是一种在复杂生物环境中定量检测ATP的可靠、灵敏和选择性的传感器，而且可以有效地输送到活细胞或组织中，并在体内作为一种信号开启传感器，对目标生物分子进行特定的、高对比度的成像。我们的设计为未来基于碳纳米材料的双光子荧光探针的开发提供了方法学模型方案，用于体外或体内测定生物或生物相关物种。
Two-photon excitation (TPE) with near-infrared (NIR) photons as the excitation source have the unique properties of lower tissue autofluorescence and self-absorption, reduced photodamage and photobleaching, higher spatial resolution, and deeper penetration depth (>500 μm). Carbon nanomaterials, for example, graphene oxide (GO), have the advantages of good biocompatibility, efficient transporters into cells, protecting the carried DNA or peptides from enzymatic cleavage, and super fluorescence quenching efficiency. By combination of the nanostructured carbon materials with the TPE technique, herein we have designed an aptamer-two-photon dye (TPdye)/GO TPE fluorescent nanosensing conjugate for molecular probing in biological fluids, living cells, and zebrafish. This approach takes advantage of the exceptional quenching capability of GO for the proximate TP dyes and the higher affinity of single-stranded DNA on GO than the aptamer-target complex. Successful in vitro and in vivo detection of ATP was demonstrated with this sensing strategy. Our results reveal that the GO/Aptamer-TPdye system not only is a robust, sensitive, and selective sensor for quantitative detection of ATP in the complex biological environment but also can be efficiently delivered into live cells or tissues and act as a "signal-on" in vivo sensor for specific, high-contrast imaging of target biomolecules. Our design provides a methodology model scheme for development of future carbon nanomaterial-based two-photon fluorescent probes for in vitro or in vivo determination of biological or biologically relevant species.