The polysaccharide scaffold of PrP 27-30 is a common compound of natural prions and consists of α-linked polyglucose
The polysaccharide scaffold of PrP 27-30 is a common compound of natural prions and consists of α-linked polyglucose
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DOI：
10.1515/bc.2005.131
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发表时间：
2005-11-01
影响因子：
3.7
通讯作者：
Riesner, D
中科院分区：
文献类型：
--
作者：
Dumpitak, C;Beekes, M;Riesner, D
鉴定为朊病毒棒（PrP 27-30）的5-15%组分的惰性多糖支架与PrP的N-糖基基团和GPI锚明确区分，并且主要由1，4-连接的葡萄糖组成，具有经由1，4，6-连接的葡萄糖的一些分支。我们表明，这种多糖支架是一种常见的二级组成部分的朊病毒中发现仓鼠全长PrPSc，朊病毒棒和小鼠ScN 2a朊病毒从细胞培养。从朊病毒棒的制备得到了改进，导致多糖支架没有剩余的感染性。此外，我们确定了糖苷键的立体化学主要是（如果不是完全）α-糖苷键。多糖的起源，它与PrP的相互作用及其与糖原和淀粉体的潜在关系进行了讨论。
An inert polysaccharide scaffold identified as a 5-15% component of prion rods (PrP 27-30) is unambiguously distinguishable from the N-glycosyl groups and the GPI anchor of PrP, and consists predominantly of 1,4-linked glucose with some branching via 1,4,6-linked glucose. We show that this polysaccharide scaffold is a common secondary component of prions found in hamster fulllength PrPSc, prion rods and in mouse ScN2a prions from cell culture. The preparation from prion rods was improved, resulting in a polysaccharide scaffold free of remaining infectivity. Furthermore, we determined the stereochemistry of the glycoside linkages as predominantly if not entirely alpha-glycosidic. The origin of the polysaccharide, its interaction with PrP and its potential relation to glycogen and corpora amylacea are discussed.