Diagnosis of enterovirus brain disease in hypogammaglobulinemic patients by polymerase chain reaction.
Diagnosis of enterovirus brain disease in hypogammaglobulinemic patients by polymerase chain reaction.
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通过聚合酶链反应诊断低丙种球蛋白血症患者肠道病毒脑病。
DOI：
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发表时间：
1993
影响因子：
11.8
通讯作者：
N. Hyman
中科院分区：
文献类型：
--
作者：
A. Webster;H. Rotbart;T. Warner;P. Rudge;N. Hyman
采用基于聚合酶链反应（PCR）扩增病毒基因组序列的技术，从3例原发性低丙种球蛋白血症和慢性脑部疾病患者中采集的CSF样本对肠道病毒RNA呈阳性。反复尝试从这些患者的CSF中培养病毒均未成功，可能是因为患者定期接受静脉注射免疫球蛋白治疗，其中可能含有足够的特异性抗体以部分中和病毒。我们的数据表明，肠道病毒是负责不同的中枢神经系统功能的患者原发性低丙种球蛋白血症，这些患者的诊断应采用PCR技术。
CSF samples taken from three patients with primary hypogammaglobulinemia and chronic brain disease were positive for enterovirus RNA by use of a technique based on the polymerase chain reaction (PCR) to amplify viral genomic sequences. Repeated attempts to culture viruses from the CSF from these patients were unsuccessful, possibly because the patients were treated regularly with intravenous immunoglobulin, which may have contained enough specific antibody to partially neutralize the viruses. Our data suggest that enteroviruses are responsible for diverse CNS features in patients with primary hypogammaglobulinemia and that for these patients the diagnosis should be pursued using PCR technology.
特发性心肌病和心肌炎肠道病毒诊断的分子方法。
影响因子：
24
作者：
Tracy,S;Wiegand,V;McManus,B;Gauntt,C;Pallansch,M;Beck,M;Chapman,N
通讯作者：
Chapman,N