CALCIUM-DEPENDENT PRIMING OF DNA SYNTHESIS IN ISOLATED RAT LIVER NUCLEI
CALCIUM-DEPENDENT PRIMING OF DNA SYNTHESIS IN ISOLATED RAT LIVER NUCLEI
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DOI：
10.1016/0006-291x(70)90786-2
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发表时间：
1970-01-01
影响因子：
3.1
通讯作者：
ATKINSON, MR
中科院分区：
文献类型：
--
作者：
BURGOYNE, LA;WAGAR, MA;ATKINSON, MR
从大鼠肝脏中分离的细胞核在没有Ca ~（2+）的情况下将脱氧核糖核苷酸掺入到添加的poly d（A-T）中，但不掺入到其具有高分子量的核DNA中。在暴露于Ca 2+时，核DNA被切割，然后在Mg 2+存在下，通过核DNA聚合酶的作用，作为掺入脱氧核糖核苷酸的引物。链终止阿拉伯糖类似物araCMP 1的掺入是细胞核中DNA引物位点的Ca 2+依赖性产生的量度。
Nuclei isolated from rat liver in the absence of Ca2+incorporate deoxyribonucleotides into added poly d(A-T) but not into their nuclear DNA, which is of high molecular weight. On exposure to Ca2+the nuclear DNA is nicked and then serves as primer for incorporation of deoxyribonucleotides, in the presence of Mg2+, through the action of nuclear DNA polymerase. Incorporation of the chain-terminating arabinosyl analog araCMP1is a measure of Ca2+-dependent production of DNA primer sites in nuclei.