Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol
Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol
复制标题
DOI：
10.1038/nbt1352
复制
发表时间：
2007-11-01
影响因子：
46.9
通讯作者：
Chen, Xiao-Ya
中科院分区：
文献类型：
--
作者：
Mao, Ying-Bo;Cai, Wen-Juan;Chen, Xiao-Ya
我们从棉铃虫（Helicoverpa armigera）中鉴定出一个细胞色素P450基因（CYP6AE14），该基因允许这种草食性动物耐受棉花代谢物棉酚的抑制浓度。当棉酚被添加到饲料中时，CYP6AE14在中肠中高表达，其表达与幼虫生长相关。当幼虫被喂食表达CYP6AE14特异性双链RNA （dsRNA）的植物材料时，该转录物在中肠中的水平降低，幼虫生长迟缓。在棉酚的存在下，这两种效果都更为显著。由于谷胱甘肽- s -转移酶基因（GST1）在表达GST1 dsRNA的植物中处于沉默状态，因此给昆虫喂食表达dsRNA的植物材料可能是触发RNA干扰的一种通用策略，在昆虫学研究和害虫现场防治中具有应用价值。
We identify a cytochrome P450 gene (CYP6AE14) from cotton bollworm (Helicoverpa armigera), which permits this herbivore to tolerate otherwise inhibitory concentrations of the cotton metabolite, gossypol. CYP6AE14 is highly expressed in the midgut and its expression correlates with larval growth when gossypol is included in the diet. When larvae are fed plant material expressing double-stranded RNA (dsRNA) specific to CYP6AE14, levels of this transcript in the midgut decrease and larval growth is retarded. Both effects are more dramatic in the presence of gossypol. As a glutathione-S-transferase gene (GST1) is silenced in GST1 dsRNA-expressing plants, feeding insects plant material expressing dsRNA may be a general strategy to trigger RNA interference and could find applications in entomological research and field control of insect pests.