High-Throughput Method of Whole-Brain Sectioning, Using the Tape-Transfer Technique.
High-Throughput Method of Whole-Brain Sectioning, Using the Tape-Transfer Technique.
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DOI：
10.1371/journal.pone.0102363
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Mitra PP
中科院分区：
文献类型：
--
作者：
Pinskiy V;Jones J;Tolpygo AS;Franciotti N;Weber K;Mitra PP
Cryostat sectioning is a popular but labor-intensive method for preparing histological brain sections. We have developed a modification of the commercially available CryoJane tape collection method that significantly improves the ease of collection and the final quality of the tissue sections. The key modification involves an array of UVLEDs to achieve uniform polymerization of the glass slide and robust adhesion between the section and slide. This report presents system components and detailed procedural steps, and provides examples of end results; that is, 20μm mouse brain sections that have been successfully processed for routine Nissl, myelin staining, DAB histochemistry, and fluorescence. The method is also suitable for larger brains, such as rat and monkey.
