Golgi biogenesis in Toxoplasma gondii
Golgi biogenesis in Toxoplasma gondii
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DOI：
10.1038/nature00946
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发表时间：
2002-08-01
期刊：
影响因子：
64.8
通讯作者：
Warren, G
中科院分区：
文献类型：
--
作者：
Pelletier, L;Stern, CA;Warren, G
两种模型已经被提出来解释新的高尔基体在细胞周期中的生长。第一个结果表明，一个新的高尔基体通过从头合成从内质网中生长出来（1）。第二个表明，一个预先存在的高尔基体是需要一个新的生长，也就是说，高尔基体是一个自主复制的细胞器（2）。为了解决这个问题，我们利用了顶复门寄生虫弓形虫的简单性（3），它只有一个高尔基体（4）。在这里，我们表明，通过使用视频荧光显微镜和三维重建的系列薄切片，高尔基体生长的过程中的横向扩展，然后由内侧分裂。进一步的分裂导致一对高尔基体结构的每个子体的遗传，然后它们结合以重新形成单个高尔基体。我们的研究结果表明，新的高尔基体通过自主复制生长，并提出了高尔基体是类似于中心粒的配对结构的可能性（5）。
Two models have been put forward to explain the growth of new Golgi during the cell cycle. The first suggests that a new Golgi grows out of the endoplasmic reticulum by de novo synthesis(1). The second suggests that a pre-existing Golgi is needed for the growth of a new one, that is, the Golgi is an autonomously replicating organelle(2). To resolve this issue, we have exploited the simplicity of the apicomplexan parasite Toxoplasma gondii(3), which has only a single Golgi stack(4). Here we show, by using video fluorescence microscopy and three-dimensional reconstructions of serial thin sections, that the Golgi grows by a process of lateral extension followed by medial fission. Further fission leads to the inheritance by each daughter of a pair of Golgi structures, which then coalesce to re-form a single Golgi. Our results indicate that new Golgi grow by autonomous duplication and raise the possibility that the Golgi is a paired structure that is analogous to centrioles(5).