[Protective effect of notoginsenoside R1 on neuron injury induced by OGD/R through ATF6/Akt signaling pathway].
[Protective effect of notoginsenoside R1 on neuron injury induced by OGD/R through ATF6/Akt signaling pathway].
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DOI：
10.19540/j.cnki.cjcmm.20170121.014
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发表时间：
2017-03-01
期刊：
影响因子：
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通讯作者：
Wang, Sha-Li
中科院分区：
文献类型：
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作者：
Hou, Qian-Ling;Wang, Yan;Wang, Sha-Li
三七皂苷R1（NGR 1）是传统中药三七中的一种重要化合物，是一种雌激素受体激动剂，具有抗凋亡、抗氧化、抗炎等作用，被广泛应用于多种疾病的治疗。本研究采用原代皮层神经元建立氧-葡萄糖剥夺/复氧（OGD/R）损伤模型。分别用NGR 1和雌激素受体抑制剂ICI-182780处理后，采用MTT法、乳酸脱氢酶（LDH）法和Hoechst 33342染色法检测神经元存活率、细胞膜完整性和细胞凋亡，采用Western blotting法检测ATF 6 α、p-Akt、Akt、Bax和Cleaved Caspase-3蛋白表达水平。结果表明，与空白对照组相比，OGD/R可诱导细胞损伤和凋亡（P
Notoginsenoside R1(NGR1),a critical compound in traditional herb Panax notoginseng, is a kind of estrogen receptor agonist.It is reported to exhibit anti-apoptotic,anti-oxidative and anti-inflammatory properties activity, so it is widely used for treatment of various diseases.In order to investigate the potential neuroprotective effect of NGR1 in hypoxic-ischemic brain damage(HIBD), primary cortical neurons were used in this study to establish oxygen-glucose deprivation/reoxygenation(OGD/R) injury models. They were treated with NGR1 and estrogen receptor inhibitor ICI-182780 respectively, then the neuronal survival, cell membrane integrity and apoptosis were assessed by MTT assay,lactate dehydrogenase test(LDH) and Hoechst 33342 stain respectively, while the protein expression levels of ATF6alpha,p-Akt,Akt,Bax and Cleaved Caspase-3 were measured by Western blotting. Results indicated that as compared with the blank control group,OGD/R could induce cell injury and apoptosis(P