THE ETS DOMAIN PROTEIN POINTED-P2 IS A TARGET OF MAP KINASE IN THE SEVENLESS SIGNAL-TRANSDUCTION PATHWAY
THE ETS DOMAIN PROTEIN POINTED-P2 IS A TARGET OF MAP KINASE IN THE SEVENLESS SIGNAL-TRANSDUCTION PATHWAY
复制标题
DOI：
10.1038/370386a0
复制
发表时间：
1994-08-04
期刊：
影响因子：
64.8
通讯作者：
KLAMBT, C
中科院分区：
文献类型：
--
作者：
BRUNNER, D;DUCKER, K;KLAMBT, C
果蝇发育中眼睛中R7感光细胞的命运由Sevenless（Sev）受体酪氨酸激酶控制（1，2）。Sev激活涉及蛋白质Ras 1和Raf以及卷曲/促分裂原活化蛋白（R1/hIAP）激酶的高度保守的信号转导级联（3）。在这里，我们表明，由P2转录的点（pnt）基因编码的ETS结构域蛋白是一个核目标的RI/MAP激酶的下游行为的信号级联反应。PntP 2蛋白在体外被R1/MAP激酶在单个位点磷酸化，并且该位点是其体内功能所需的。此外，我们目前的遗传和生化数据表明，MAP激酶控制神经发育通过磷酸化的两个拮抗转录因子的ETS家族，严和PntP 2。
The fate of the R7 photoreceptor cell in the developing eye of Drosophila is controlled by the Sevenless (Sev) receptor tyrosine kinase(1,2). Sev activates a highly conserved signal transduction cascade involving the proteins Ras1 and Raf and the Rolled/mitogen-activated protein (Rl/hlAP) kinase(3). Here we show that the ETS domain protein encoded by the P2 transcript of the pointed (pnt) gene is a nuclear target of this signalling cascade which acts downstream of RI/MAP kinase. The PntP2 protein is phosphorylated by Rl/MAP kinase in vitro at a single site and this site is required for its function in vivo. Furthermore, we present genetic and biochemical data suggesting that MAP kinase controls neural development through phosphorylation of two antagonizing transcription factors of the ETS family, Yan and PntP2.