Can Early Failure of Cartilage Implants Be Detected with Ferumoxytol Labeling?
Can Early Failure of Cartilage Implants Be Detected with Ferumoxytol Labeling?
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可以用 Ferumoxytol 标记检测软骨植入物的早期失效吗？
DOI：
10.1148/radiol.2019190793
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发表时间：
2019
期刊：
影响因子：
19.7
通讯作者：
Potter,HollisG
中科院分区：
文献类型：
--
作者：
Sneag,DarrylB;Potter,HollisG
植入后第2周，ferumoxytol标记的凋亡MASI显示铁信号丢失，表现为T2延长（26.6 msec ± 4.9），而ferumoxytol标记的活MASI显示显著较低的T2值（20.8 msec ± 5.3; P=. 001）。凋亡马西组2周时铁的丢失与软骨修复不完全相关，这一点得到了12-24周时组织病理学结果的支持。一个潜在的研究局限是诱导凋亡的方式--即使用丝裂霉素。丝裂霉素诱导的细胞凋亡是一种人工过程，在速率和机制方面可能与细胞植入后自然发生的细胞凋亡不同。另一种方法是放置没有细胞或冷冻的非活细胞的纤维蛋白胶，但据推测，作者的目标是模拟植入活细胞后可能发生的情况，然后进行凋亡。作者还提到MASI的凋亡细胞被巨噬细胞清除，但在提供的组织学结果中未显示任何巨噬细胞。巨噬细胞可能在组织修复和再生中发挥核心作用，并且过度的巨噬细胞积累与导致纤维化的细胞因子产生相关。在接受具有凋亡细胞的MASI的那些组中，愈合受损可能更多地是由于巨噬细胞的不良作用，而不是植入细胞的损失。
2nd week following implantation, ferumoxytol-labeled apoptotic MASIs exhibited loss of iron signal, manifesting as T2 prolongation (26.6 msec 6 4.9), while ferumoxytol-labeled viable MASIs demonstrated significantly lower T2 values (20.8 msec 6 5.3; P=. 001). Loss of iron at 2 weeks in the apoptotic MASI group correlated with incomplete chondral repair, supported by histopathologic findings at 12–24 weeks.A potential study limitation was the way in which apoptosis was induced—that is, with the use of mitomycin. Mitomycininduced apoptosis is an artificial process that likely differs, with regard to the rate and mechanism, from naturally occurring apoptosis after cell implantation. An alternative method would be placement of fibrin glue without cells or with frozen nonviable cells, but presumably the authors’ goal was to model what might ensue following implantation of viable cells that then undergo apoptosis. The authors also mention that apoptotic cells of MASIs are cleared by macrophages, but do not demonstrate any macrophages in the histologic results provided. Macrophages likely do play a central role in tissue repair and regeneration, and excessive macrophage accumulation is associated with cytokine production that leads to fibrosis. It is possible that impaired healing in those groups receiving MASIs with apoptotic cells was due more to an adverse effect of macrophages rather than to the loss of implanted cells.
使用 Ferumoxytol 增强 MRI 追踪小型猪软骨缺损中的干细胞植入物。
影响因子：
19.7
作者：
Theruvath,AshokJ;Nejadnik,Hossein;Lenkov,Olga;Yerneni,Ketan;Li,Kai;Kuntz,Lara;Wolterman,Cody;Tuebel,Jutta;Burgkart,Rainer;Liang,Tie;Felt,Stephen;Daldrup-Link,HeikeE
通讯作者：
Daldrup-Link,HeikeE