Ion-transporting capacity and aerobic respiration of larval white seabass (Atractoscion nobilis) may be resilient to ocean acidification conditions
Ion-transporting capacity and aerobic respiration of larval white seabass (Atractoscion nobilis) may be resilient to ocean acidification conditions
复制标题
白鲈幼体（Atractoscion nobilis）的离子传输能力和有氧呼吸可能对海洋酸化条件具有抵抗力
DOI：
10.1016/j.scitotenv.2021.148285
复制
发表时间：
2021
影响因子：
9.8
通讯作者：
Tresguerres, Martin
中科院分区：
文献类型：
--
作者：
Kwan, Garfield T.;Shen, Sara G.;Drawbridge, Mark;Checkley, David M.;Tresguerres, Martin
海洋酸化（OA）已被提议以牺牲幼鱼生长为代价来增加对酸碱调节的能量需求。在这里，白色鲈鱼（学名：Aesthoscion nobilis）的卵和幼虫被饲养在控制（542 ± 28 μatm）和升高pCO 2（1831 ± 105 μatm），直到受精后5天（dpf）。通过Na+/K+-ATP酶（NKA）的免疫检测来鉴定皮肤离子细胞。与对照组相比，暴露于pCO 2升高的幼虫具有显著较高的皮肤离子细胞数量和密度。然而，当离子细胞的大小占，相对离子细胞面积（总离子调节能力的代理）是不变的。同样，有相对NKA丰度，休息O2消耗率，和总长度之间的控制和治疗幼虫在5 dpf，也没有在2和5 dpf之间的相对离子细胞面积和总长度变化率的差异。总之，我们的研究结果表明，预计为下一个世纪OA条件不显着影响的ionoregulatory能力或能量消耗的幼虫白色鲈鱼。最后，对饲养亲鱼的循环水族箱系统中的水进行的追溯分析显示，在本实验之前，父母已经暴露于平均约1200 μatm的二氧化碳中至少3.5年。未来的研究应调查是否幼虫白色鲈鱼自然弹性OA，或者如果这种弹性是由于父母的慢性驯化OA，和/或自然选择在elevatedpCO 2产卵和受精。
Ocean acidification (OA) has been proposed to increase the energetic demand for acid-base regulation at the expense of larval fish growth. Here, white seabass (Atractoscion nobilis) eggs and larvae were reared at control (542 ± 28 μatm) and elevatedpCO2(1831 ± 105 μatm) until five days post-fertilization (dpf). Skin ionocytes were identified by immunodetection of the Na+/K+-ATPase (NKA) enzyme. Larvae exposed to elevatedpCO2possessed significantly higher skin ionocyte number and density compared to control larvae. However, when ionocyte size was accounted for, the relative ionocyte area (a proxy for total ionoregulatory capacity) was unchanged. Similarly, there were no differences in relative NKA abundance, resting O2consumption rate, and total length between control and treatment larvae at 5 dpf, nor in the rate at which relative ionocyte area and total length changed between 2 and 5 dpf. Altogether, our results suggest that OA conditions projected for the next century do not significantly affect the ionoregulatory capacity or energy consumption of larval white seabass. Finally, a retroactive analysis of the water in the recirculating aquarium system that housed the broodstock revealed the parents had been exposed to averagepCO2of ~1200 μatm for at least 3.5 years prior to this experiment. Future studies should investigate whether larval white seabass are naturally resilient to OA, or if this resilience is the result of parental chronic acclimation to OA, and/or from natural selection during spawning and fertilization in elevatedpCO2.