Pharmacological Strategies for Manipulating Plant Ca(2+) Signalling.

Pharmacological Strategies for Manipulating Plant Ca(2+) Signalling.
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DOI:
10.3390/ijms19051506
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发表时间:
2018-05-18
影响因子:
5.6
通讯作者:
Vanneste S
Vanneste S
中科院分区:
生物学2区
文献类型:
--
作者:
De Vriese K;Costa A;Beeckman T;Vanneste S

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钙是所有生物体中最具多效性的第二信使之一。然而,信号传导特异性是通过时空调节的签名编码的,其以手术精度起作用以引起高度特异性的细胞反应。如何实现这一点在植物领域仍然是一个巨大的挑战,部分原因是缺乏特定的工具来操纵/询问植物Ca 2+工具包。在许多情况下,研究人员求助于在动物细胞中优化的工具。然而,植物和动物之间明显较大的进化距离意味着观察到的效应很可能不是特定于预期的植物目标。在这里,我们提供了一个概述的药理学策略,通常用于激活或抑制植物Ca 2+信号。我们专注于在可能的情况下突出行动模式,并警告潜在的陷阱。总之,这篇评论旨在指导植物研究人员通过钙+药理学沼泽。
Calcium is one of the most pleiotropic second messengers in all living organisms. However, signalling specificity is encoded via spatio-temporally regulated signatures that act with surgical precision to elicit highly specific cellular responses. How this is brought about remains a big challenge in the plant field, in part due to a lack of specific tools to manipulate/interrogate the plant Ca2+ toolkit. In many cases, researchers resort to tools that were optimized in animal cells. However, the obviously large evolutionary distance between plants and animals implies that there is a good chance observed effects may not be specific to the intended plant target. Here, we provide an overview of pharmacological strategies that are commonly used to activate or inhibit plant Ca2+ signalling. We focus on highlighting modes of action where possible, and warn for potential pitfalls. Together, this review aims at guiding plant researchers through the Ca2+ pharmacology swamp.
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