Recombinant Dengue Type 2 Viruses with Altered E Protein Domain III Epitopes Are Efficiently Neutralized by Human Immune Sera
Recombinant Dengue Type 2 Viruses with Altered E Protein Domain III Epitopes Are Efficiently Neutralized by Human Immune Sera
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DOI：
10.1128/jvi.06871-11
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发表时间：
2012-01
影响因子：
5.4
通讯作者：
W. Wahala;C. Huang;S. Butrapet;L. White;A. D. de Silva
中科院分区：
文献类型：
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作者：
W. Wahala;C. Huang;S. Butrapet;L. White;A. D. de Silva
摘要登革病毒（DENV）感染后，人类产生多克隆、型特异性中和抗体应答。中和DENV的许多小鼠抗体结合病毒包膜（EDIII）蛋白的结构域III上的侧脊或A链表位。已经假定这些表位也是人中和抗体的主要靶标。使用具有改变的EDIII表位的重组登革血清2型病毒，我们证明EDIII表位不是人中和抗体的主要靶标。
ABSTRACT Humans develop polyclonal, serotype-specific neutralizing antibody responses after dengue virus (DENV) infection. Many mouse antibodies that neutralize DENV bind to the lateral ridge or A strand epitopes on domain III of the viral envelope (EDIII) protein. It has been assumed that these epitopes are also the main target of human neutralizing antibodies. Using recombinant dengue serotype 2 viruses with altered EDIII epitopes, we demonstrate that EDIII epitopes are not the main target of human neutralizing antibody.