A CAPTIVE BUBBLE METHOD REPRODUCES THE INSITU BEHAVIOR OF LUNG SURFACTANT MONOLAYERS
A CAPTIVE BUBBLE METHOD REPRODUCES THE INSITU BEHAVIOR OF LUNG SURFACTANT MONOLAYERS
复制标题
DOI：
10.1152/jappl.1989.67.6.2389
复制
发表时间：
1989-12-01
影响因子：
3.3
通讯作者：
POSSMAYER, F
中科院分区：
文献类型：
--
作者：
SCHURCH, S;BACHOFEN, H;POSSMAYER, F
我们用兔肺表面活性物质和牛肺表面活性物质脂提取物水悬浮液中的吸附膜和从溶剂中涂布的二棕榈酰-SN-3-甘油磷酰胆碱(DPPC)膜，测试了一种新的捕获型气泡表面张力计。由于没有管子穿透气泡表面，消除了表面膜在外压作用下被压缩的潜在泄漏通道。表面张力和面积直接根据气泡的形状计算，而不需要测量压力。只需一到两次压缩，兔表面活性剂膜就表现出低表面张力、低塌陷速率和低可压缩性，这是扩散DPPC膜的特性。之前Pattle(Proc.)描述的气泡“点击”现象R.Soc.朗德。B Biol.SCI。148：217-240,1958)也被复制，但仅用牛肺泡抽提物的原位表面进行复制，在表面试验中表现不如兔表面活性物质。这些结果表明，表面活性物质载脂蛋白A可能存在于兔而不是牛的制剂中，它增强了肺表面活性物质单层的吸附和稳定性。
We tested a new captive bubble surface tensiometer with films adsorbed from aqueous suspensions of rabbit lung surfactant and a bovine lung surfactant lipid extract and with films of dipalmitoyl-sn-3-glycerophosphorylcholine (DPPC) spread from solvents. The lack of tubes penetrating the bubble surface eliminated potential leakage pathways for the surface film, which was compressed by increasing external pressure. Surface tensions and areas were calculated directly from bubble shapes without the need of pressure measurements. After only one to two compressions, the rabbit surfactant films exhibited the low surface tension, collapse rates, and compressibilities characteristic of the behavior of spread DPPC films. The bubble "clicking" phenomenon described earlier by Pattle (Proc. R. Soc. Lond. B Biol. Sci. 148: 217-240, 1958) was also reproduced, but only with the bovine extract alveolar surface in situ and approached the which did not perform as well as the rabbit surfactant in surface tests. These findings suggest that surfactant apoprotein SP-A, which was probably present in the rabbit but not the bovine preparations, enhances both adsorption and stability of pulmonary surfactant monlayers.