Structural Inheritance of the Actin Cytoskeletal Organization Determines the Body Axis in Regenerating Hydra
Structural Inheritance of the Actin Cytoskeletal Organization Determines the Body Axis in Regenerating Hydra
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DOI：
10.1016/j.celrep.2017.01.036
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发表时间：
2017-02-07
期刊：
影响因子：
8.8
通讯作者：
Keren, Kinneret
中科院分区：
文献类型：
--
作者：
Livshits, Anton;Shani-Zerbib, Lital;Keren, Kinneret
了解力学如何补充生物信号在形态发生过程中定义模式是一个突出的挑战。在这里，我们利用多细胞水螅的肌动球蛋白细胞骨架的形态发生中的作用进行调查。我们发现超细胞肌动蛋白纤维组织是从父母水螅遗传的，并决定了再生组织片段的体轴。这种形式的结构遗传是不平凡的，因为组织折叠和动态肌动蛋白重组参与。我们进一步表明，多个身体轴的出现可以追溯到肌动蛋白纤维排列在再生过程的早期阶段的差异。锚定再生九头蛇硬线上引起的机械约束抑制了多个身体轴的出现，突出了机械反馈的重要性，在定义和稳定的身体轴。总之，这些结果构成了一个重要的一步，发展的形态发生，结合力学的综合观点。
Understanding how mechanics complement biosignaling in defining patterns during morphogenesis is an outstanding challenge. Here, we utilize the multicellular polyp Hydra to investigate the role of the actomyosin cytoskeleton in morphogenesis. We find that the supra- cellular actin fiber organization is inherited from the parent Hydra and determines the body axis in regenerating tissue segments. This form of structural inheritance is non- trivial because of the tissue folding and dynamic actin reorganization involved. We further show that the emergence of multiple body axes can be traced to discrepancies in actin fiber alignment at early stages of the regeneration process. Mechanical constraints induced by anchoring regenerating Hydra on stiff wires suppressed the emergence of multiple body axes, highlighting the importance of mechanical feedbacks in defining and stabilizing the body axis. Together, these results constitute an important step toward the development of an integrated view of morphogenesis that incorporates mechanics.