Vanadate and phosphate ions reduce tension and increase cross-bridge kinetics in chemically skinned heart muscle.
Vanadate and phosphate ions reduce tension and increase cross-bridge kinetics in chemically skinned heart muscle.
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钒酸盐和磷酸盐离子可降低化学剥皮心肌的张力并增加跨桥动力学。
DOI：
10.1016/0304-4165(81)90392-5
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Solaro,RJ
中科院分区：
文献类型：
--
作者：
Herzig,JW;Peterson,JW;Rüegg,JC;Solaro,RJ
在含有不同浓度的磷酸盐（Pi）或钒酸盐（主要是H2 VO 4 −，pH 7）离子的溶液中，测量化学皮肤心肌条的张力发展、即时刚度和ATP酶。钒酸盐和Pid使硬度与张力成比例地降低。结果表明，与Pi一样，钒酸盐加速跨桥的周转率，但在Pi效应所需浓度的约1/500时有效。Pi和钒酸盐均增加了等长张力维持的能量消耗（即ATP酶与张力的比率），并增加了化学皮肤心肌条快速拉伸后延迟张力发展的速度。结果还表明，在拉伸激活过程中的延迟张力的上升速率的变化可能反映的变化的生化循环的跨桥的动力学。
Tension development, immediate stiffness and ATPase of chemically skinned myocardial strips were measured in solutions with varying concentrations of phosphate (Pi) or vanadate (predominantly H2VO4−at pH 7) ion. Vanadate and Pidecreased stiffness in proportion to tension. The results show that, like Pi, vanadate accelerates the turnover rate of cross-bridges, but is effective at about 1/500 the concentration required for the Pieffect. Both Piand vanadate increased the energy cost of isometric tension maintenance (that is, the ratio of ATPase to tension) and increased the velocity of delayed tension development following quick stretch of the chemically skinned myocardial strips. The results also show that changes in the rate of rise of delayed tension during stretch activation probably reflect changes in the kinetics of the biochemical cycle of the cross-bridges.