Knockout of MIMP protein in lactobacillus plantarum lost its regulation of intestinal permeability on NCM460 epithelial cells through the zonulin pathway.
Knockout of MIMP protein in lactobacillus plantarum lost its regulation of intestinal permeability on NCM460 epithelial cells through the zonulin pathway.
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植物乳杆菌中 MIMP 蛋白的敲除失去了通过连蛋白途径对 NCM460 上皮细胞肠道通透性的调节作用
DOI：
10.1186/1471-230x-14-171
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发表时间：
2014-10-03
影响因子：
2.4
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Liu Z;Kang L;Li C;Tong C;Huang M;Zhang X;Huang N;Moyer MP;Qin H;Wang J
背景以往的研究表明，植物乳杆菌CGMCC 1258整合膜蛋白中的微整合膜蛋白对肠上皮损伤具有保护作用。本研究通过建立微整合膜蛋白敲除植物乳杆菌(LPKM)，观察其保护作用的变化，验证微整合膜蛋白对正常肠屏障功能的保护作用。方法采用体外结合实验和肠道通透性实验，验证微整合膜蛋白对肠道通透性的保护作用。分子机制也被确定为带状蛋白途径。结果LPKM对EPEC与NCM460细胞黏附的抑制作用减弱。LPKM对肠源性大肠埃希菌引起的肠道通透性下降的缓解作用较弱，对肠源性大肠杆菌引起的zonlin表达增加的拮抗作用较弱。过表达zonrin可降低植物乳杆菌调节的肠道通透性。血清佐菌素水平与术后败血症呈正相关。因此，微整合膜蛋白可能是植物乳杆菌对肠屏障保护作用所必需的。
BackgroundPrevious studies indicated that the micro integral membrane protein located within the media place of the integral membrane protein of Lactobacillus plantarum CGMCC 1258 had protective effects against the intestinal epithelial injury. In our study, we mean to establish micro integral membrane protein -knockout Lactobacillus plantarum (LPKM) to investigate the change of its protective effects and verify the role of micro integral membrane protein on protection of normal intestinal barrier function.MethodsBinding assay and intestinal permeability were performed to verify the protective effects of micro integral membrane protein on intestinal permeability in vitro and in vivo. Molecular mechanism was also determined as the zonulin pathway. Clinical data were also collected for further verification of relationship between zonulin level and postoperative septicemia.ResultsLPKM got decreased inhibition of EPEC adhesion to NCM460 cells. LPKM had lower ability to alleviate the decrease of intestinal permeability induced by enteropathogenic-e.coli, and prevent enteropathogenic-e.coli -induced increase of zonulin expression. Overexpression of zonulin lowered the intestinal permeability regulated by Lactobacillus plantarum. There was a positive correlation between zonulin level and postoperative septicemia. Therefore, micro integral membrane protein could be necessary for the protective effects of Lactobacillus plantarum on intestinal barrier.Conclusion