New isocysteine building blocks and chemoselective peptide ligation.
New isocysteine building blocks and chemoselective peptide ligation.
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新的异半胱氨酸结构单元和化学选择性肽连接。
DOI：
10.1039/b309235f
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发表时间：
2004
影响因子：
3.2
通讯作者：
O. Seitz
中科院分区：
文献类型：
--
作者：
C. Dose;O. Seitz
BOC、Fmoc和CBZ保护的异半胱氨酸构建块是通过从硫代马来酸开始的简明的三步过程制备的。使用Boc/Trt保护的异半胱氨酸提供了方便地获得异半胱氨酸多肽的途径，从而允许化学选择性地连接水中未保护的多肽片段。比较了异半胱氨酸介导的结扎与半胱氨酸介导的天然化学结扎的pH依赖性。
Boc-, Fmoc- and Cbz-protected isocysteine building blocks were prepared by a concise three-step procedure starting from thiomalic acid. The use of Boc/Trt-protected isocysteine provided convenient access to isocysteinyl peptides that allow the chemoselective ligation of unprotected peptide fragments in water. The pH-dependency of the isocysteine-mediated ligation was compared with that of cysteine-mediated native chemical ligation.
影响因子：
15
作者：
Hondal, RJ;Nilsson, BL;Raines, RT
通讯作者：
Raines, RT