Comparisons between Geographically Diverse Samples of Carried Staphylococcus aureus
Comparisons between Geographically Diverse Samples of Carried Staphylococcus aureus
复制标题
DOI：
10.1128/jb.00493-09
复制
发表时间：
2009-09-15
影响因子：
3.2
通讯作者：
Feil, Edward J.
中科院分区：
文献类型：
--
作者：
Ruimy, Raymond;Armand-Lefevre, Laurence;Feil, Edward J.
大约三分之一的人口被金黄色葡萄球菌无症状地定植。然而，运输种群内的大部分全球多样性仍然没有特征，也不清楚这种变异在多大程度上是地理上的分割。我们同时从法国、阿尔及利亚、摩尔多瓦和柬埔寨四个国家的1531名成年人中分离出300株卡介菌。对所有菌株进行多位点序列分型。在所有四个样品(CC30、-45、-121、-15、-5和-8)中均存在6个克隆复合体(CC)。基于基因频率的分析显示，法国和阿尔及利亚的样本最相似，柬埔寨的样本最不同。虽然这种模式与可能的人类迁移率和地理距离一致，但随机克隆扩张也导致了地区差异。系统发育分析显示，柬埔寨境内存在一个高度分化且没有特征的基因(ST1223)。这一血统与CC75有关，以前只有在澳大利亚北部偏远的土著人口中才能观察到CC75。
Approximately one-third of the human population is asymptomatically colonized by Staphylococcus aureus. However, much of the global diversity within the carriage populations remains uncharacterized, and it is unclear to what degree the variation is geographically partitioned. We isolated 300 carriage isolates from 1,531 adults contemporaneously in four countries: France, Algeria, Moldova, and Cambodia. All strains were characterized by multilocus sequence typing. Six clonal complexes (CCs) were present in all four samples (CC30, -45, -121, -15, -5, and -8). Analyses based on the genotype frequencies revealed the French and Algerian samples to be most similar and the Cambodian sample to be most distinct. While this pattern is consistent with likely rates of human migration and geographic distance, stochastic clonal expansion also contributes to regional differences. Phylogenetic analysis revealed a highly divergent and uncharacterized genotype (ST1223) within Cambodia. This lineage is related to CC75, which has previously been observed only in remote aboriginal populations in northern Australia.