Effects of intraocular irrigation with melphalan on rabbit retinas during vitrectomy
Effects of intraocular irrigation with melphalan on rabbit retinas during vitrectomy
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DOI：
10.1007/s00417-007-0685-3
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发表时间：
2008-04-01
影响因子：
2.7
通讯作者：
Kaneko, Akihiro
中科院分区：
文献类型：
--
作者：
Shimoda, Yukitoshi;Hamano, Rika;Kaneko, Akihiro
目的探讨玻璃体切割术中玻璃体内灌注马法兰对家兔视网膜的毒性作用。方法对18只健康白化兔的18只眼在玻璃体切除术中眼内灌注5、10、20μg/ml浓度的马法兰进行视网膜电图检查(ERG)。接受玻璃体切除术但未使用马法兰的另一只眼睛作为对照。每组两只兔子均观察视网膜组织病理学变化。结果5μg/ml灌注组术后28天ERG和组织学与对照眼相比无明显变化。在10和20μg/ml组中，平均a波振幅分别降至对侧眼的52%和31%；平均 b 波幅度分别下降至 52% 和 19%。然而，术后28天期间，10μg/ml组和20μg/ml组的a波和b波隐式峰值时间没有显着差异。组织学切片显示10μg/ml组的内核层坏死和外核层变薄。 20μg/ml组观察到外核层和感光层丢失以及内核层坏死。结论玻璃体切除术中玻璃体内灌注5μg/ml美法仑对视网膜无毒。这种治疗方式可能是治疗玻璃体种植视网膜母细胞瘤的潜在方法。
Purpose To investigate the toxic effects of perfusion of intravitreal melphalan during vitrectomy on the rabbit retina.Methods We performed electoretinography (ERG) in 18 eyes of 18 healthy albino rabbits before and after intraocular melphalan perfusion at concentrations of 5-, 10-, and 20-mu g/ml during pars plana vitrectomy. Fellow eyes that underwent vitrectomy without melphalan served as controls. The histopathologic retinal changes were observed in both eyes of two rabbits from each group.Results In the 5-mu g/ml perfusion group, the ERGs and histology showed no substantial changes compared with control fellow eyes during 28 days postoperatively. In the 10- and 20-mu g/ml groups, the mean a-wave amplitude decreased to 52% and 31% respectively of the fellow eye; the mean b-wave amplitude decreased to 52% and 19% respectively. However, the peak implicit time of the a- and b-waves did not significantly differ in the 10- and 20-mu g/ml groups during 28 days postoperatively. Histologic sections showed necrosis of the inner nuclear layer and thinning of the outer nuclear layer in the 10-mu g/ml group. Loss of the outer nuclear layer and the photoreceptor layer and necrosis of the inner nuclear layer were observed in the 20-mu g/ml group.Conclusion The intravitreal 5-mu g/ml melphalan perfusion during vitrectomy appears to be nontoxic to the retina. This therapeutic modality might be a potential treatment for retinoblastoma with vitreous seeding.