Estrous cycle dependence of nitrosomethylurea (NMU)-induced preneoplastic lesions in rat mammary gland.
Estrous cycle dependence of nitrosomethylurea (NMU)-induced preneoplastic lesions in rat mammary gland.
复制标题
亚硝基甲基脲（NMU）诱导的大鼠乳腺肿瘤前病变的动情周期依赖性。
DOI：
10.1016/0304-3835(91)90197-p
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发表时间：
1991
期刊：
影响因子：
9.7
通讯作者：
Beattie,CW
中科院分区：
文献类型：
--
作者：
Anderson,CH;Hussain,RA;Han,MC;Beattie,CW
处女50-55日龄大鼠表现出有规律的发情周期，静脉注射。在发情前期、发情期或发情间期的早晨使用直接作用致癌物1-亚硝基-1-甲基脲(NMU)。在NMU给药后每周一次处死大鼠，以确定显微镜下可识别的不典型增生的来源和数量。NMU给药后1周内出现端芽异常，动情前期(PE)和动情间期(E)注射组大鼠乳腺TEB异常数目明显多于发情间期(DE)组。在注射NMU后3周出现导管(DH)、导管肺泡增生症(DAH)和增生性肺泡结节(HAN)，在注射NMU后6周以上各型病变的发生率明显增加。在动情期注射的大鼠的腺体中，汉族人最多。腺癌发生于近端和远端导管网络；在NMU后10周和12周，动情前期注射的大鼠肿瘤明显多于动情间期和动情期。这些结果支持NMU给药时荷尔蒙环境显著改变大鼠乳腺肿瘤早期发展的理论。
Virgin 50–55-day-old rats exhibiting regular estrous cycles were injected i.v. with the direct acting carcinogen 1-nitroso-1-methylurea (NMU) on the morning of proestrus, estrus, or diestrus. Rats were killed at weekly intervals following NMU administration to identify source and number of microscopically identifiable dysplasias. Terminal end bud (TEB) abnormalities appeared within 1 week following NMU administration, with a significantly greater number of abnormal TEBs in mammary glands of rats injected on proestrus (PE) and estrus (E) than on diestrus (DE). Ductal (DH) and ductal alveolar hyperplasias (DAH) and hyperplastic alveolar nodules (HAN) appeared during week 3, with significantly more of each type of lesion appearing by 6 weeks after NMU injection. HAN were most numerous in glands from rats injected on estrus. Adenocarcinomas arose from both the proximal and distal ductal network; at 10 and 12 weeks post NMU, significantly more tumors were found in rats injected on proestrus than diestrus and estrus. These results support the theory that the hormonal environment at the time of NMU administration significantly alters early development of mammary tumors in the rat.