HUMAN MUSCLE MACROPHAGES EXPRESS BETA-AMYLOID PRECURSOR AND PRION PROTEINS AND THEIR MESSENGER-RNAS
HUMAN MUSCLE MACROPHAGES EXPRESS BETA-AMYLOID PRECURSOR AND PRION PROTEINS AND THEIR MESSENGER-RNAS
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DOI：
10.1097/00001756-199505090-00024
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发表时间：
1995-05-09
期刊：
影响因子：
1.7
通讯作者：
ENGEL, WK
中科院分区：
文献类型：
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作者：
ASKANAS, V;SARKOZI, E;ENGEL, WK
利用具有良好特性的抗β-淀粉样前体蛋白(β-APP)和蛋白(PrP)抗体和特异性cRNA探针，对人肌肉巨噬细胞中的β-APP和PrP及其mRNAs进行了定位。51例各种神经肌肉疾病患者肌肉活检组织中的巨噬细胞显示βAPP和PrP积聚，并强烈表达βAPP和PrP mRNAs。这些都存在于所有肌肉巨噬细胞中，与它们在肌肉组织中的定位或诊断无关。我们的研究首次证明了人类肌肉停留的巨噬细胞合成并积累了β-APP和PrP。我们认为这些蛋白在肌肉巨噬细胞的生物学中发挥作用，包括它们参与炎症和免疫反应。
WELL-CHARACTERIZED antibodies against beta-amyloid precursor protein (beta APP) and prion protein (PrP), and specific cRNA probes, were used to localize beta APP and PrP and their mRNAs in human muscle macrophages. Macrophages present in muscle biopsies of 51 patients with various neuromuscular disorders showed accumulation of beta APP and PrP, and strongly expressed beta APP and PrP mRNAs. These were present in all muscle macrophages unrelated to their localization within the muscle tissue or diagnosis. Our study provides the first demonstration that human muscle resident macrophages synthesize and accumulate beta APP and PrP. We suggest that those proteins play a role in biology of muscle macrophages, including their participation in inflammatory and immune responses.