Seed size plasticity in response to embryonic lethality conferred by ectopic CYCD activation is dependent on plant architecture.
Seed size plasticity in response to embryonic lethality conferred by ectopic CYCD activation is dependent on plant architecture.
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DOI：
10.1080/15592324.2016.1192741
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发表时间：
2016-07-02
影响因子：
2.9
通讯作者：
Murray JA
中科院分区：
文献类型：
--
作者：
Sornay E;Dewitte W;Murray JA
种子的大小是细胞在胚、胚乳和珠被三个种子隔室中增殖和生长的结果。将D型细胞周期蛋白CYCD 7;1靶向表达到中央细胞和早期胚乳（FWA：CYCD 7;1）引发核分裂和部分胚珠败育，减少每个角果中的种子数量并导致种子大小增加。在其他分离胚致死突变中观察到对种子大小的类似影响，这表明在解释表观种子大小表型时需要谨慎。在这里，我们表明FWA：CYCD 7;1对拟南芥种子大小的积极影响受到母株结构的调节。在具有未修饰花序的FWA：CYCD 7;1系中产生较大的种子，并且在去除侧枝和腋生茎后也产生较大的种子。这种表型是不存在的花序与增加腋生花茎修剪的主茎。鉴于这种明显的混杂影响资源分配的转基因效果，我们得出结论，植物结构是一个更重要的因素，考虑在评价种子表型。
The size of seeds is the result of cell proliferation and growth in the three seed compartments: the embryo, endosperm and integuments. Targeting expression of the D-type cyclin CYCD7;1 to the central cell and early endosperm (FWA:CYCD7;1) triggered nuclear divisions and partial ovule abortion, reducing seed number in each silique and leading to increased seed size. A similar effect on seed size was observed with other segregating embryo lethal mutations, suggesting caution is needed in interpreting apparent seed size phenotypes. Here, we show that the positive effect of FWA:CYCD7;1 on Arabidopsis seed size is modulated by the architecture of the mother plant. Larger seeds were produced in FWA:CYCD7;1 lines with unmodified inflorescences, and also upon removal of side branches and axillary stems. This phenotype was absent from inflorescences with increased axillary floral stems produced by pruning of the main stem. Given this apparent confounding influence of resource allocation on transgenes effect, we conclude that plant architecture is a further important factor to consider in appraising seed phenotypes.