Ex vivo implications of phytohormones on various in vitro responses in Leptadenia reticulata (Retz.) Wight. & Arn.—An endangered plant
Ex vivo implications of phytohormones on various in vitro responses in Leptadenia reticulata (Retz.) Wight. & Arn.—An endangered plant
复制标题
DOI：
10.1016/j.envexpbot.2010.05.009
复制
发表时间：
2013-02
影响因子：
5.7
通讯作者：
M. S. Rathore;M. S. Rathore;N. S. Shekhawat
中科院分区：
文献类型：
--
作者：
M. S. Rathore;M. S. Rathore;N. S. Shekhawat
Leptadenia reticulata (Retz.) Wight。 ＆阿恩。是一种重要的药用植物，属于萝藦科。自公元前 4500 年以来，这种植物就因其药用用途而闻名。目前，这是一种濒临灭绝的物种（Arya et al., 2003）。每个节点有 6 个芽（2-4 厘米长）在 MS 培养基+5.0mg/l BAP+添加剂上分化。在培养基中加入添加剂促进了培养物的生长。将每个外植体分化的芽重复转移到新鲜的MS+1.0mg/l的BAP+0.1mg/l的NAA和添加剂上。间隔3周后，定期在MS+1.0mg/l BAP+0.5mg/l Kin+2-iP（0.5mg/l）和0.1mg/l NAA+添加剂上对再生的芽进行传代培养以进一步增殖。在培养基中添加硫酸铵导致枝条数量增加并促进伸长，即使继代培养被延迟（26±2天），培养物的生长也能持续。在确定从叶柄衍生的愈伤组织芽体外再生的方案方面也取得了成功。 15-20天后，通过这两个过程在体外再生的芽在1/4浓度的MS培养基+3.0mg/l IBA的体外生根。如果用 200mg/l IBA 处理体外再生芽，则体外生根的百分比。体外-离体生根的幼苗在温室中不同的温度和湿度条件下硬化。将硬化的小植株转移到塑料袋中的土壤中。超过 95% 的植物在田间条件下存活。年收获的总干生物量为2800公斤/英亩。
Leptadenia reticulata (Retz.) Wight. & Arn. is an important medicinal plant, belongs to the family Asclepiadaceae. This plant is known for its medicinal uses since 4500 BC. Presently this is an endangered species (Arya et al., 2003). Six shoots (2–4cm long) per node differentiated on MS medium+5.0mg/l of BAP+additives. Incorporation of additives in the culture medium promoted growth of cultures. The shoots differentiated per explant were repeatedly transferred on to fresh MS+1.0mg/l of BAP+0.1mg/l of NAA and additives. The regenerated shoots were subcultured for further multiplication on MS+1.0mg/l BAP+0.5mg/l Kin+2-iP (0.5mg/l) and 0.1mg/l of NAA+additives regularly after an interval of 3 weeks. Addition of ammonium sulphate in the medium resulted in increase in shoot number and promoted elongation also growth of cultures was sustained even if subculturing was delayed (26±2 days). Success was also achieved in defining protocol for in vitro regeneration of shoots from petiole derived callus. Shoots regenerated in vitro by both processes were rooted in vitro on 1/4 strength of MS medium+3.0mg/l of IBA after 15–20 days. Cent percent of the shoots rooted ex vitro, if the in vitro regenerated shoots were treated with 200mg/l of IBA. The in vitro–ex vitro rooted plantlets were hardened under different regimes of temperature and humidity in a greenhouse. The hardened plantlets were transferred to soil in polybags. More than 95% plants survived in field conditions. Total dry biomass harvested per year was 2800kg/acre.