Ultrashort Echo Time T1ρ Is Sensitive to Enzymatic Degeneration of Human Menisci.
Ultrashort Echo Time T1ρ Is Sensitive to Enzymatic Degeneration of Human Menisci.
复制标题
超短回波时间 T1Ï 对人类半月板的酶促变性敏感。
DOI：
10.1097/rct.0000000000000265
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发表时间：
2015
影响因子：
1.3
通讯作者：
Chung,ChristineB
中科院分区：
文献类型：
--
作者：
Chang,EricY;Campos,JulianaC;Bae,WonC;Znamirowski,Richard;Statum,Sheronda;Du,Jiang;Chung,ChristineB
目的研究超短回波时间(UTE)-T1ρ磁共振（MR）定量测量对胰蛋白酶消化人半月板蛋白多糖降解的敏感性。方法采用3T扫描仪对4例半月板标本进行常规和定量UTE-T1ρ MR序列分析。在胰蛋白酶溶液浸泡4,8和12小时之前和之后进行磁共振成像，诱导蛋白多糖损失。一个样本作为对照。分析消化液中糖胺聚糖（GAG）的含量。分析UTE-T1ρ研究的定量变化。结果图像表现为进行性组织肿胀，纤维紊乱，GAG消失后信号强度增加。胰蛋白酶治疗后UTE-T1ρ值有随时间升高的趋势（P= 0.06）。经胰蛋白酶处理的样品的累积GAG损失呈增加趋势（P= 0.1）。结论短回波时间T1ρ测量可以无创性地检测和量化半月板退变的严重程度，而半月板退变与骨关节炎的进展有关。
ObjectiveThe aim of the study was to determine whether quantitative ultrashort echo time (UTE)-T1ρ magnetic resonance (MR) measurements are sensitive to proteoglycan degradation in human menisci by trypsin digestion.MethodsConventional and quantitative UTE-T1ρ MR sequences were performed on 4 meniscal samples using a 3T scanner. Magnetic resonance imaging was performed before and after 4, 8, and 12 hours of trypsin solution immersion, inducing proteoglycan loss. One sample was used as a control. Digest solutions were analyzed for glycosaminoglycan (GAG) content. The UTE-T1ρ studies were analyzed for quantitative changes.ResultsImages showed progressive tissue swelling, fiber disorganization, and increase in signal intensity after GAG depletion. The UTE-T1ρ values tended to increase with time after trypsin treatment (P= 0.06). Cumulative GAG loss into the bath showed a trend of increased values for trypsin-treated samples (P= 0.1).ConclusionsUltrashort echo time T1ρ measurements can noninvasively detect and quantify severity of meniscal degeneration, which has been correlated with progression of osteoarthritis.