Escherichia coli Isolates from Broiler Chicken Meat, Broiler Chickens, Pork, and Pigs Share Phylogroups and Antimicrobial Resistance with Community-Dwelling Humans and Patients with Urinary Tract Infection
Escherichia coli Isolates from Broiler Chicken Meat, Broiler Chickens, Pork, and Pigs Share Phylogroups and Antimicrobial Resistance with Community-Dwelling Humans and Patients with Urinary Tract Infection
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DOI：
10.1089/fpd.2009.0409
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发表时间：
2010-05-01
影响因子：
2.8
通讯作者：
Hammerum, Anette M.
中科院分区：
文献类型：
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作者：
Jakobsen, Lotte;Kurbasic, Azra;Hammerum, Anette M.
大肠杆菌是泌尿道感染（UTI）最常见的原因。系统群B2和D分离株与UTI相关。有人提出E.引起UTI的大肠杆菌可能是动物源性的。本研究的目的是调查大肠埃希氏菌群和抗菌药物耐药性，以及它们之间可能的联系。从丹麦的UTI患者、社区居民、肉鸡肉、肉鸡、猪肉和猪中分离的大肠杆菌。共收集到964个地理和时间上匹配的E.对来自UTI患者（n = 102）、社区居民（n = 109）、丹麦（n = 197）和进口肉鸡肉（n = 86）、丹麦肉鸡（n = 138）、丹麦（n = 177）和进口猪肉（n = 10）以及丹麦猪（n = 145）的大肠杆菌分离株进行了大肠杆菌群检测（A、B1、B2、D和不可分型[NT]分离株）和抗菌药物敏感性。除进口猪源分离株外，其余各组分离株均为A、B1、B2、D和NT系统群。对三种（B2分离株）或五种抗菌药物（A、B1、D和NT分离株）的抗菌药物耐药性在分离株中共享，无论其来源如何。使用聚类分析来调查抗菌素耐药性数据，我们发现UTI分离株总是与来自肉类和/或动物的分离株分组。我们检测到B2和D分离株，这是与UTI，从肉鸡，肉鸡，猪肉和猪的分离株。虽然B2分离株在动物和肉类中的流行率较低，但这些来源仍可能构成获得尿路致病性大肠杆菌的风险。杆菌此外，E.从动物和肉类中分离的大肠杆菌与UTI分离株的耐药性表型非常相似。因此，我们的研究为以下假设提供了支持：食用动物和肉类可能存在引起UTI的E。杆菌
Escherichia coli is the most common cause of urinary tract infection (UTI). Phylogroup B2 and D isolates are associated with UTI. It has been proposed that E. coli causing UTI could have an animal origin. The objective of this study was to investigate the phylogroups and antimicrobial resistance, and their possible associations in E. coli isolates from patients with UTI, community-dwelling humans, broiler chicken meat, broiler chickens, pork, and pigs in Denmark. A total of 964 geographically and temporally matched E. coli isolates from UTI patients (n = 102), community-dwelling humans (n = 109), Danish (n = 197) and imported broiler chicken meat (n = 86), Danish broiler chickens (n = 138), Danish (n = 177) and imported pork (n = 10), and Danish pigs (n = 145) were tested for phylogroups (A, B1, B2, D, and nontypeable [NT] isolates) and antimicrobial susceptibility. Phylogroup A, B1, B2, D, and NT isolates were detected among all groups of isolates except for imported pork isolates. Antimicrobial resistance to three (for B2 isolates) or five antimicrobial agents (for A, B1, D, and NT isolates) was shared among isolates regardless of origin. Using cluster analysis to investigate antimicrobial resistance data, we found that UTI isolates always grouped with isolates from meat and/or animals. We detected B2 and D isolates, that are associated to UTI, among isolates from broiler chicken meat, broiler chickens, pork, and pigs. Although B2 isolates were found in low prevalences in animals and meat, these sources could still pose a risk for acquiring uropathogenic E. coli. Further, E. coli from animals and meat were very similar to UTI isolates with respect to their antimicrobial resistance phenotype. Thus, our study provides support for the hypothesis that a food animal and meat reservoir might exist for UTI-causing E. coli.