Vesicular stomatitis virus vectors expressing avian influenza H5HA induce cross-neutralizing antibodies and long-term protection
Vesicular stomatitis virus vectors expressing avian influenza H5HA induce cross-neutralizing antibodies and long-term protection
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DOI：
10.1016/j.virol.2007.04.021
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发表时间：
2007-09-15
期刊：
影响因子：
3.7
通讯作者：
Rose, John K.
中科院分区：
文献类型：
--
作者：
Schwartz, Jennifer A.;Buonocore, Linda;Rose, John K.
鉴于H5 N1禽流感病毒（AIV）的致命性和从家禽到人类的反复传播，可能需要针对H5 N1病毒的有效疫苗来预防大流行。我们产生了实验疫苗载体的基础上重组水泡性口炎病毒（VSV）表达H5血凝素（HA）从1997年分离的H5 N1病毒。HA基因从减毒的野生型VSV载体或从含有VSV G基因缺失的单循环载体表达。我们发现，所有的载体诱导有效的中和抗体滴度对同源和抗原异源H5 N1病毒在2004年和2005年分离。用初免或初免/加强载体的任何组合接种小鼠提供了针对AIV攻击的持久保护（> 7个月），即使在接受单剂量单循环疫苗的动物中也是如此。我们的数据表明，这些重组体是有希望的大流行性流感疫苗候选者。(c)2007年爱思唯尔公司All rights reserved.
Given the lethality of H5N1 avian influenza viruses (AIV) and the recurring spread from poultry to humans, an effective vaccine against H5N1 viruses may be needed to prevent a pandemic. We generated experimental vaccine vectors based on recombinant vesicular stomatitis virus (VSV) expressing the H5 hemagglutinin (HA) from an H5N1 virus isolated in 1997. The HA gene was expressed either from an attenuated wild-type VSV vector or from a single-cycle vector containing a deletion of the VSV G gene. We found that all of the vectors induced potent neutralizing antibody titers against the homologous and antigenically heterologous H5N1 viruses isolated in 2004 and 2005. Vaccination of mice with any combination of prime or prime/boost vectors provided long-lasting protection (> 7 months) against challenge with AIV, even in animals receiving a single dose of single-cycle vaccine. Our data indicate that these recombinants are promising vaccine candidates for pandemic influenza. (c) 2007 Elsevier Inc. All rights reserved.