Induction of stress metabolites in immobilized Glycyrrhiza echinata cultured cells
Induction of stress metabolites in immobilized Glycyrrhiza echinata cultured cells
复制标题
固定化甘草培养细胞应激代谢物的诱导
DOI：
10.1007/bf00269114
复制
发表时间：
1986
影响因子：
6.2
通讯作者：
T. Furuya
中科院分区：
文献类型：
--
作者：
S. Ayabe;K. Iida;T. Furuya
将培养的松果甘草细胞转移到新鲜培养基中或通过海藻酸钙凝胶包埋进行固定，会导致细胞和培养基中的逆查耳酮、松果素迅速而短暂地积累。与自由悬浮的细胞相比，固定化细胞中观察到的松果素产生水平更高且持续时间更长。将细胞转移到含有海藻酸钠或氯化钙的培养基中，并将海藻酸钠添加到悬浮培养物中，引起与细胞固定中观察到的相同的效果。在诱导细胞中还检测到了一种新的代谢物。通过固定细胞，参与松果素生物合成的酶的活性迅速增加。
Transfer into a fresh medium or immobilization by entrapment in calcium alginate gels of cultured Glycyrrhiza echinata cells caused a rapid and transient accumulation of a retrochalcone, echinatin, in both the cells and the medium. The higher level and longer duration of echinatin production was observed in the immobilized cells than in freely suspended cells. Transfer of the cells into the medium containing either sodium alginate or calcium chloride, and the addition of sodium alginate into the suspension culture, caused the same effect as observed in cell immobilization. A novel metabolite was also detected in the induced cells. Activities of the enzymes involved in echinatin biosynthesis were shown to rapidly increase by immobilization of the cells.