A new aptamer/SWNTs IDE-SPQC sensor for rapid and specific detection of Group A Streptococcus
A new aptamer/SWNTs IDE-SPQC sensor for rapid and specific detection of Group A Streptococcus
复制标题
一种新型适体/单壁碳纳米管 IDE-SPQC 传感器，用于快速、特异性检测 A 组链球菌
DOI：
10.1016/j.snb.2014.03.067
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发表时间：
2014-07-01
影响因子：
8.4
通讯作者：
Zhang, Xiaoqing
中科院分区：
文献类型：
--
作者：
Shi, Xiaohong;He, Fengjiao;Zhang, Xiaoqing
快速、准确地检测A组链球菌（GAS）感染对临床诊断具有重要意义。本文构建了一种新的适配体/单壁碳纳米管叉指型金电极压电传感器，用于检测气体。以所筛选的抗GAS适体为识别探针，以单壁碳纳米管（SWNTs）为电子传感器，固定在叉指型金电极（IDE）上，串联压电石英晶体（SPQC）。该传感器的响应机制是基于GAS特异性地与固定在单壁碳纳米管IDE上的抗GAS适体反应，使适体从单壁碳纳米管IDE上脱离，从而导致IDE-SPQC的灵敏频移响应。传感器的频移与GAS浓度在3 × 10（6）cfu/ml ~ 3 × 10（6）cfu/ml范围内呈线性关系。检测限为12 cfu/ml。检测时间小于40 min。使用构建的传感器检测牛奶中GAS的回收率为76-97%。该方法可用于临床诊断和环境气体的快速检测。(C)2014爱思唯尔有限公司版权所有。
Rapid and accurate detection of Group A Streptococcus (GAS) infection is very important in clinical diagnosis. In this paper, a new aptamer/SWNTs interdigital gold electrode piezoelectric sensor was constructed for detection of GAS. The selected anti-GAS aptamer was used as recognition probe and single-walled carbon nanotubes (SWNTs) immobilized on the Interdigital Gold Electrode (IDE) was used as electron transducer which connected to piezoelectric quartz crystal (SPQC) in series. The response mechanism of constructed sensor was based on the fact that GAS specifically reacted with anti-GAS aptamer immobilized on SWNTs IDE and caused aptamer detaching from SWNTs IDE and resulted sensitively frequency shift response of IDE-SPQC. The frequency shift of constructed sensor had a linear relationship with the concentration of GAS in the range of 3 Chi 10(6) cfu/ml to 3 Chi 10(6) cfu/ml. The detection limit was 12 cfu/ml GAS. The detection time was less than 40 min. The recovery rate for detection of GAS in milk was 76-97% using constructed sensor. It would find application in clinical diagnosis and environment for rapid detection of GAS. (C) 2014 Elsevier B.V. All rights reserved.