Failure to detect prion protein (PrPres) by immunohistochemistry in striated muscle tissues of animals experimentally inoculated with agents of transmissible spongiform encephalopathy
Failure to detect prion protein (PrPres) by immunohistochemistry in striated muscle tissues of animals experimentally inoculated with agents of transmissible spongiform encephalopathy
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DOI：
10.1354/vp.41-1-78
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发表时间：
2004-01-01
影响因子：
2.4
通讯作者：
Cutlip, RC
中科院分区：
文献类型：
--
作者：
Hamir, AN;Miller, JM;Cutlip, RC
传染性海绵状脑病是一种致命的神经系统疾病。由病原体，朊病毒感染，诱导一种异常形式的朊病毒蛋白（Prp(res)）在神经和淋巴系统组织中积累。存在特征性的组织病理学改变（海绵状脑病）和在神经和淋巴组织中检测蛋白酶抗性Prp（res）是目前诊断tse的基础方法。本研究采用免疫组化（IHC）方法对20只动物（牛、羊、麋鹿和浣熊）的横纹肌组织（舌、心、膈肌和咬肌）进行了PrPres检测。实验接种后，所有动物均发生TSE。通过免疫组化在所有被检查的动物的大脑中发现了PrPres，但在肌肉组织中没有发现。这些发现与最近发表的关于患tse的实验动物的报告相矛盾，在这些报告中，在舌头和其他横纹肌中检测到这些改变的朊病毒蛋白。需要进一步的肌肉组织测试来证实本研究的发现。
Transmissible spongiform encephalopathies (TSEs) are fatal neurologic diseases. Infection by the causative agent, a prion, induces accumulations of an abnormal form of prion protein (Prp(res)) in tissues of nervous and lymphoid systems. Presence of characteristic histopathologic changes (spongiform encephalopathy) and detection of protease-resistant Prp(res) in neural and lymphoid tissues are the basis of currently available methods for diagnosis of TSEs. In this study, samples of striated muscle tissues (tongue, heart, diaphragm, and masseter muscle) from 20 animals (cattle, sheep, elk, and raccoons) were examined for PrPres by immunohistochemistry (IHC). All the animals had developed a TSE after experimental inoculation. PrPres was found by IHC in the brain but not in the muscle tissues of all the animals examined. These findings are contradictory to recently published reports of laboratory animals with TSEs, where these altered prion proteins Were detected in tongue and other striated muscles. Further testing of muscle tissues is needed to confirm the findings of the present study.